Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR

Bernardino Machado-Moreira; Silvia Monteiro; Ricardo Santos; Antonio Martinez-Murcia; Andreja Rajkovic; Nada Smigic; Karl G. Richards; Florence Abram; Catherine M. Burgess

doi:10.1016/j.mimet.2019.105786

Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR

Bernardino Machado-Moreira
, Silvia Monteiro
, Ricardo Santos
, Antonio Martinez-Murcia
, Andreja Rajkovic
, Nada Smigic
, Karl G. Richards
, Florence Abram
, Catherine M. Burgess

Sustainable World

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

4 Citations (Scopus)

Abstract

There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.

Original language	English
Article number	105786
Journal	Journal of Microbiological Methods
Volume	168
DOIs	https://doi.org/10.1016/j.mimet.2019.105786
Publication status	Published - Jan 2020

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10.1016/j.mimet.2019.105786

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title = "Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR",

abstract = "There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.",

author = "Bernardino Machado-Moreira and Silvia Monteiro and Ricardo Santos and Antonio Martinez-Murcia and Andreja Rajkovic and Nada Smigic and Richards, \{Karl G.\} and Florence Abram and Burgess, \{Catherine M.\}",

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T1 - Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR

AU - Machado-Moreira, Bernardino

AU - Monteiro, Silvia

AU - Santos, Ricardo

AU - Martinez-Murcia, Antonio

AU - Rajkovic, Andreja

AU - Smigic, Nada

AU - Richards, Karl G.

AU - Abram, Florence

AU - Burgess, Catherine M.

PY - 2020/1

Y1 - 2020/1

N2 - There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.

AB - There is increasing interest in methodologies for the simultaneous concentration and detection of multiple targets in individual samples. The aim of this study was to investigate the potential presence of E. coli DNA in beef extract powder used as part of a procedure to concentrate water samples for the simultaneous detection of bacteria, viruses and protozoa. DNA from E. coli was detected in five out of six beef extract lots tested, demonstrating the limitations of its inclusion when being used in assays that will be used for the detection of E. coli in water samples. Further work is required to clarify if this phenomenon also occurs for other microorganisms of interest in water.

UR - https://www.scopus.com/pages/publications/85075734123

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DO - 10.1016/j.mimet.2019.105786

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VL - 168

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 105786

ER -

Impact of beef extract used for sample concentration on the detection of Escherichia coli DNA in water samples via qPCR

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