@inbook{a8a40c105fb8444884db38bc6c1eb8c6,
title = "Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH)",
abstract = "This chapter describes a method used to assay the cell cycle dynamics of the centromeric histone H3 variant CENP-A in meiosis using Drosophila males as the experimental system. Specifically, we describe a method that combines Immunofluorescence (IF) and Fluorescence in-situ Hybridization (FISH) protocols, performed on fixed Drosophila testes. An advantage of this protocol is the ability to localize individual centromeres on the four Drosophila homologous chromosomes that form distinct nuclear territories in spermatocytes. We also describe a method to quantify CENP-A focal intensities using Image J software.",
keywords = "CENP-A, Centromere, Chromosome segregation, Drosophila melanogaster, Meiosis, Prophase I, Spermatogenesis",
author = "Collins, \{Caitr{\'i}ona M.\} and Dunleavy, \{Elaine M.\}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8663-7\_18",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "327--337",
booktitle = "Methods in Molecular Biology",
}