HPLC purification of adenoviral vectors

Marc Eglon, Barry McGrath, Timothy O'Brien

Research output: Chapter in Book or Conference Publication/ProceedingChapterpeer-review

2 Citations (Scopus)

Abstract

Adenoviruses are attractive vectors for gene therapy where short-term transgene expression is required. In order to meet the clinical requirements of adenovirus for use beyond the laboratory, advanced methods are required for the purification and quantitation of recombinant adenoviral vectors (rAd). Chromatographic systems offer the advantages of linear scalability and reproducibility, and this method describes a laboratory-scale process based on liquid chromatography, which can be technically transferred and readily scaled-up according to the demands of the laboratory or clinic in which it will be used.

Original languageEnglish
Title of host publicationAdvanced Protocols in Oxidative Stress II
PublisherHumana Press Inc.
Pages395-408
Number of pages14
ISBN (Print)9781607614104
DOIs
Publication statusPublished - 2010

Publication series

NameMethods in Molecular Biology
Volume594
ISSN (Print)1064-3745

Keywords

  • Adenovirus
  • Anion exchange
  • Chromatography
  • Gel filtration
  • Gene therapy vectors

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