HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at Centromeres

Elaine M. Dunleavy; Danièle Roche; Hideaki Tagami; Nicolas Lacoste; Dominique Ray-Gallet; Yusuke Nakamura; Yataro Daigo; Yoshihiro Nakatani; Geneviève Almouzni-Pettinotti

doi:10.1016/j.cell.2009.02.040

HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at Centromeres

Elaine M. Dunleavy, Danièle Roche, Hideaki Tagami, Nicolas Lacoste, Dominique Ray-Gallet, Yusuke Nakamura, Yataro Daigo, Yoshihiro Nakatani, Geneviève Almouzni-Pettinotti

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

523 Citations (Scopus)

Abstract

The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation. In the absence of an underlying DNA sequence, it is still unclear how CENP-A deposition at centromeres is determined. Here, we purified non-nucleosomal CENP-A complexes to identify direct CENP-A partners involved in such a mechanism and identified HJURP. HJURP was not detected in H3.1- or H3.3-containing complexes, indicating its specificity for CENP-A. HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP-A deposition. Furthermore, HJURP downregulation leads to a major reduction in CENP-A at centromeres and impairs deposition of newly synthesized CENP-A, causing mitotic defects. We conclude that HJURP is a key factor for CENP-A deposition and maintenance at centromeres.

Original language	English
Pages (from-to)	485-497
Number of pages	13
Journal	Cell
Volume	137
Issue number	3
DOIs	https://doi.org/10.1016/j.cell.2009.02.040
Publication status	Published - 1 May 2009
Externally published	Yes

Keywords

CELLBIO
CELLCYLE

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10.1016/j.cell.2009.02.040

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@article{12020d84cac84c56b71774c1baa07782,

title = "HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at Centromeres",

abstract = "The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation. In the absence of an underlying DNA sequence, it is still unclear how CENP-A deposition at centromeres is determined. Here, we purified non-nucleosomal CENP-A complexes to identify direct CENP-A partners involved in such a mechanism and identified HJURP. HJURP was not detected in H3.1- or H3.3-containing complexes, indicating its specificity for CENP-A. HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP-A deposition. Furthermore, HJURP downregulation leads to a major reduction in CENP-A at centromeres and impairs deposition of newly synthesized CENP-A, causing mitotic defects. We conclude that HJURP is a key factor for CENP-A deposition and maintenance at centromeres.",

keywords = "CELLBIO, CELLCYLE",

author = "Dunleavy, \{Elaine M.\} and Dani{\`e}le Roche and Hideaki Tagami and Nicolas Lacoste and Dominique Ray-Gallet and Yusuke Nakamura and Yataro Daigo and Yoshihiro Nakatani and Genevi{\`e}ve Almouzni-Pettinotti",

year = "2009",

month = may,

day = "1",

doi = "10.1016/j.cell.2009.02.040",

language = "English",

volume = "137",

pages = "485--497",

journal = "Cell",

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publisher = "Elsevier B.V.",

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T1 - HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at Centromeres

AU - Dunleavy, Elaine M.

AU - Roche, Danièle

AU - Tagami, Hideaki

AU - Lacoste, Nicolas

AU - Ray-Gallet, Dominique

AU - Nakamura, Yusuke

AU - Daigo, Yataro

AU - Nakatani, Yoshihiro

AU - Almouzni-Pettinotti, Geneviève

PY - 2009/5/1

Y1 - 2009/5/1

N2 - The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation. In the absence of an underlying DNA sequence, it is still unclear how CENP-A deposition at centromeres is determined. Here, we purified non-nucleosomal CENP-A complexes to identify direct CENP-A partners involved in such a mechanism and identified HJURP. HJURP was not detected in H3.1- or H3.3-containing complexes, indicating its specificity for CENP-A. HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP-A deposition. Furthermore, HJURP downregulation leads to a major reduction in CENP-A at centromeres and impairs deposition of newly synthesized CENP-A, causing mitotic defects. We conclude that HJURP is a key factor for CENP-A deposition and maintenance at centromeres.

AB - The histone H3 variant CenH3, called CENP-A in humans, is central in centromeric chromatin to ensure proper chromosome segregation. In the absence of an underlying DNA sequence, it is still unclear how CENP-A deposition at centromeres is determined. Here, we purified non-nucleosomal CENP-A complexes to identify direct CENP-A partners involved in such a mechanism and identified HJURP. HJURP was not detected in H3.1- or H3.3-containing complexes, indicating its specificity for CENP-A. HJURP centromeric localization is cell cycle regulated, and its transient appearance at the centromere coincides precisely with the proposed time window for new CENP-A deposition. Furthermore, HJURP downregulation leads to a major reduction in CENP-A at centromeres and impairs deposition of newly synthesized CENP-A, causing mitotic defects. We conclude that HJURP is a key factor for CENP-A deposition and maintenance at centromeres.

KW - CELLBIO

KW - CELLCYLE

UR - https://www.scopus.com/pages/publications/65249129208

U2 - 10.1016/j.cell.2009.02.040

DO - 10.1016/j.cell.2009.02.040

M3 - Article

SN - 0092-8674

VL - 137

SP - 485

EP - 497

JO - Cell

JF - Cell

IS - 3

ER -

HJURP Is a Cell-Cycle-Dependent Maintenance and Deposition Factor of CENP-A at Centromeres

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Keywords

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