Abstract
Hemocyanin is a multi-functional protein located in the hemolymph (blood) of certain arthropods and molluscs. In addition to its well-defined role in oxygen transport, hemocyanin can be converted into a phenoloxidase-like enzyme. Herein, we tested the antimicrobial properties of horseshoe crab (Limulus polyphemus) hemocyanin-derived phenoloxidase reaction products using broad ranges of phenolic substrates (e.g. L-DOPA) and microbial targets (Gram-positive/negative bacteria, yeast). The enzyme-catalysed turnover of several substrates generated (by)products that reduced significantly the number of colony forming units. Microbicidal effects of hemocyanin-derived phenoloxidase were thwarted by the inhibitor phenylthiourea. Data presented here further support a role for hemocyanin in invertebrate innate immunity.
| Original language | English |
|---|---|
| Pages (from-to) | 47-51 |
| Number of pages | 5 |
| Journal | Developmental and Comparative Immunology |
| Volume | 86 |
| DOIs | |
| Publication status | Published - Sep 2018 |
| Externally published | Yes |
Keywords
- Enzyme-substrate complexes
- Horseshoe crabs
- Innate immunity
- Melanogenesis
- Multifunctional protein