TY - JOUR
T1 - Genomic epidemiology reveals multidrug resistant plasmid spread between Vibrio cholerae lineages in Yemen
AU - Lassalle, Florent
AU - Al-Shalali, Salah
AU - Al-Hakimi, Mukhtar
AU - Njamkepo, Elisabeth
AU - Bashir, Ismail Mahat
AU - Dorman, Matthew J.
AU - Rauzier, Jean
AU - Blackwell, Grace A.
AU - Taylor-Brown, Alyce
AU - Beale, Mathew A.
AU - Cazares, Adrián
AU - Al-Somainy, Ali Abdullah
AU - Al-Mahbashi, Anas
AU - Almoayed, Khaled
AU - Aldawla, Mohammed
AU - Al-Harazi, Abdulelah
AU - Quilici, Marie Laure
AU - Weill, François Xavier
AU - Dhabaan, Ghulam
AU - Thomson, Nicholas R.
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/10
Y1 - 2023/10
N2 - Since 2016, Yemen has been experiencing the largest cholera outbreak in modern history. Multidrug resistance (MDR) emerged among Vibrio cholerae isolates from cholera patients in 2018. Here, to characterize circulating genotypes, we analysed 260 isolates sampled in Yemen between 2018 and 2019. Eighty-four percent of V. cholerae isolates were serogroup O1 belonging to the seventh pandemic El Tor (7PET) lineage, sub-lineage T13, whereas 16% were non-toxigenic, from divergent non-7PET lineages. Treatment of severe cholera with macrolides between 2016 and 2019 coincided with the emergence and dominance of T13 subclones carrying an incompatibility type C (IncC) plasmid harbouring an MDR pseudo-compound transposon. MDR plasmid detection also in endemic non-7PET V. cholerae lineages suggested genetic exchange with 7PET epidemic strains. Stable co-occurrence of the IncC plasmid with the SXT family of integrative and conjugative element in the 7PET background has major implications for cholera control, highlighting the importance of genomic epidemiological surveillance to limit MDR spread.
AB - Since 2016, Yemen has been experiencing the largest cholera outbreak in modern history. Multidrug resistance (MDR) emerged among Vibrio cholerae isolates from cholera patients in 2018. Here, to characterize circulating genotypes, we analysed 260 isolates sampled in Yemen between 2018 and 2019. Eighty-four percent of V. cholerae isolates were serogroup O1 belonging to the seventh pandemic El Tor (7PET) lineage, sub-lineage T13, whereas 16% were non-toxigenic, from divergent non-7PET lineages. Treatment of severe cholera with macrolides between 2016 and 2019 coincided with the emergence and dominance of T13 subclones carrying an incompatibility type C (IncC) plasmid harbouring an MDR pseudo-compound transposon. MDR plasmid detection also in endemic non-7PET V. cholerae lineages suggested genetic exchange with 7PET epidemic strains. Stable co-occurrence of the IncC plasmid with the SXT family of integrative and conjugative element in the 7PET background has major implications for cholera control, highlighting the importance of genomic epidemiological surveillance to limit MDR spread.
UR - https://www.scopus.com/pages/publications/85173101931
U2 - 10.1038/s41564-023-01472-1
DO - 10.1038/s41564-023-01472-1
M3 - Article
SN - 2058-5276
VL - 8
SP - 1787
EP - 1798
JO - Nature Microbiology
JF - Nature Microbiology
IS - 10
ER -
