Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Min Liu; Ning Ge; Yongfeng Han; Jamie Reilly; Meimei Yang; Fan Yang; Janusz Krawczyk; Veronica McInerney; Timothy O'Brien; Terence Prendiville; Sanbing Shen

doi:10.1016/j.scr.2021.102607

Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Min Liu
, Ning Ge
, Yongfeng Han
, Jamie Reilly
, Meimei Yang
, Fan Yang
, Janusz Krawczyk
, Veronica McInerney
, Timothy O'Brien
, Terence Prendiville
, Sanbing Shen

Medicine

Hebei Normal University
University of Galway
Royal College of Surgeons in Ireland
Hebei Province Children's Hospital
Galway University Hospital
Our Ladies Hospital for Sick Children

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

Abstract

Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.

Original language	English
Article number	102607
Pages (from-to)	102607
Journal	Stem Cell Research
Volume	57
DOIs	https://doi.org/10.1016/j.scr.2021.102607
Publication status	Published - Dec 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.scr.2021.102607

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Liu, M., Ge, N., Han, Y., Reilly, J., Yang, M., Yang, F., Krawczyk, J., McInerney, V., O'Brien, T., Prendiville, T., & Shen, S. (2021). Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual. Stem Cell Research, 57, 102607. Article 102607. https://doi.org/10.1016/j.scr.2021.102607

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abstract = "Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.",

author = "Min Liu and Ning Ge and Yongfeng Han and Jamie Reilly and Meimei Yang and Fan Yang and Janusz Krawczyk and Veronica McInerney and Timothy O'Brien and Terence Prendiville and Sanbing Shen",

year = "2021",

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doi = "10.1016/j.scr.2021.102607",

language = "English",

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AU - Liu, Min

AU - Ge, Ning

AU - Han, Yongfeng

AU - Reilly, Jamie

AU - Yang, Meimei

AU - Yang, Fan

AU - Krawczyk, Janusz

AU - McInerney, Veronica

AU - O'Brien, Timothy

AU - Prendiville, Terence

AU - Shen, Sanbing

PY - 2021/12

Y1 - 2021/12

N2 - Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.

AB - Skin punch biopsy was donated by a healthy 51-year-old Caucasian male and the dermal fibroblasts were reprogrammed into human induced pluripotent stem cell (hiPSC) lines by using non-integrative Sendai viruses expressing OCT4, SOX2, KLF4 and c-MYC. Three iPSC lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) highly expressed the pluripotent markers and were capable of differentiating into cells of endodermal, mesodermal, and ectodermal origin. These iPSCs can be offered as controls and in combination with genome-editing and three-dimensional (3D) system. They may be used for human disease modelling and drug screening.

UR - https://www.scopus.com/pages/publications/85119917019

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DO - 10.1016/j.scr.2021.102607

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AN - SCOPUS:85119917019

SN - 1873-5061

VL - 57

SP - 102607

JO - Stem Cell Research

JF - Stem Cell Research

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ER -

Generation and characterization of three induced pluripotent stem cell lines (NUIGi046-A, NUIGi046-B, NUIGi046-C) from a 51-year-old healthy individual

Abstract

UN SDGs

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