Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male

Ning Ge; Min Liu; Xiaoran Zhu; Janusz Krawczyk; Veronica McInerney; Sanbing Shen; Timothy O'Brien; Terence Prendiville

doi:10.1016/j.scr.2021.102389

Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male

Ning Ge
, Min Liu
, Xiaoran Zhu
, Janusz Krawczyk
, Veronica McInerney
, Sanbing Shen
, Timothy O'Brien
, Terence Prendiville

Medicine

University of Galway
Hebei Normal University
Hebei General Hospital
Galway University Hospital
Our Ladies Hospital for Sick Children

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

Abstract

We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming.

Original language	English
Article number	102389
Journal	Stem Cell Research
Volume	53
DOIs	https://doi.org/10.1016/j.scr.2021.102389
Publication status	Published - May 2021

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10.1016/j.scr.2021.102389

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title = "Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male",

abstract = "We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming.",

author = "Ning Ge and Min Liu and Xiaoran Zhu and Janusz Krawczyk and Veronica McInerney and Sanbing Shen and Timothy O'Brien and Terence Prendiville",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

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doi = "10.1016/j.scr.2021.102389",

language = "English",

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T1 - Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male

AU - Ge, Ning

AU - Liu, Min

AU - Zhu, Xiaoran

AU - Krawczyk, Janusz

AU - McInerney, Veronica

AU - Shen, Sanbing

AU - O'Brien, Timothy

AU - Prendiville, Terence

PY - 2021/5

Y1 - 2021/5

N2 - We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming.

AB - We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming.

UR - https://www.scopus.com/pages/publications/85105756257

U2 - 10.1016/j.scr.2021.102389

DO - 10.1016/j.scr.2021.102389

M3 - Article

C2 - 34088016

AN - SCOPUS:85105756257

SN - 1873-5061

VL - 53

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102389

ER -

Generation and characterization of three induced pluripotent stem cell lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a 7-year-old healthy Caucasian male

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