Abstract
Autofluorescent proteins (AFPs) have revolutionized molecular cell biology, and applications continue to harness the power of these genetically encoded fluorescent tags. Here, we review the discovery and physical properties of AFPs as well as their development through mutational optimization for several functional parameters. A practical guide to selection and use of major AFPs is provided as well as an overview of techniques for experimental applications.Autofluorescent proteins (AFPs) have revolutionized molecular cell biology, and applications continue to harness the power of these genetically encoded fluorescent tags. Here, we review the discovery and physical properties of AFPs as well as their development through mutational optimization for several functional parameters. A practical guide to selection and use of major AFPs is provided as well as an overview of techniques for experimental applications.
| Original language | English (Ireland) |
|---|---|
| ISBN (Electronic) | 0091-679X (Print) 0091-67 |
| Publication status | Published - 1 Jan 2008 |