Fertilization of zona-free hamster ova by ionophore-treated boar sperm and the condensation of sperm chromosomes

J. M. Bird; J. A. Houghton

doi:10.1016/0378-4320(89)90123-1

Fertilization of zona-free hamster ova by ionophore-treated boar sperm and the condensation of sperm chromosomes

J. M. Bird, J. A. Houghton

Infectious Disease

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

3 Citations (Scopus)

Abstract

Porcine spermatozoa were assessed for their ability to undergo the acrosome reaction and penetrate zona-free hamster eggs following treatment with ionophore, in order to select an optimal capacitation regime prior to fertilization and culture for sperm chromosomes. Penetration rates achieved 86.5% ± 6.0 at 0.5 μM ionophore ^-BSA with only 17.3 ± 8.4 of spermatozoa remaining acrosome intact (P < 0.01) at this concentration. Sperm attachment to the plasmalemma was not related to penetration rates or induction of the acrosome reaction. Following capacitation of spermatozoa with 0.5 μM ionophore (^-BSA) and fertilization of zona-free oocytes, we obtained a hamster-porcine metaphase plate yield of 13.9 ± 4.2 at 22-25 h post-insemination. A high percentage of spreads exhibited arrested sperm heads (48.7 ± 14.7). These results demonstrate that porcine sperm chromosome metaphases may be obtained following capacitation of spermatozoa and penetration of zona-free oocytes in vitro.

Original language	English
Pages (from-to)	125-140
Number of pages	16
Journal	Animal Reproduction Science
Volume	21
Issue number	2
DOIs	https://doi.org/10.1016/0378-4320(89)90123-1
Publication status	Published - 15 Dec 1989

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title = "Fertilization of zona-free hamster ova by ionophore-treated boar sperm and the condensation of sperm chromosomes",

abstract = "Porcine spermatozoa were assessed for their ability to undergo the acrosome reaction and penetrate zona-free hamster eggs following treatment with ionophore, in order to select an optimal capacitation regime prior to fertilization and culture for sperm chromosomes. Penetration rates achieved 86.5\% ± 6.0 at 0.5 μM ionophore -BSA with only 17.3 ± 8.4 of spermatozoa remaining acrosome intact (P < 0.01) at this concentration. Sperm attachment to the plasmalemma was not related to penetration rates or induction of the acrosome reaction. Following capacitation of spermatozoa with 0.5 μM ionophore (-BSA) and fertilization of zona-free oocytes, we obtained a hamster-porcine metaphase plate yield of 13.9 ± 4.2 at 22-25 h post-insemination. A high percentage of spreads exhibited arrested sperm heads (48.7 ± 14.7). These results demonstrate that porcine sperm chromosome metaphases may be obtained following capacitation of spermatozoa and penetration of zona-free oocytes in vitro.",

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T1 - Fertilization of zona-free hamster ova by ionophore-treated boar sperm and the condensation of sperm chromosomes

AU - Bird, J. M.

AU - Houghton, J. A.

PY - 1989/12/15

Y1 - 1989/12/15

N2 - Porcine spermatozoa were assessed for their ability to undergo the acrosome reaction and penetrate zona-free hamster eggs following treatment with ionophore, in order to select an optimal capacitation regime prior to fertilization and culture for sperm chromosomes. Penetration rates achieved 86.5% ± 6.0 at 0.5 μM ionophore -BSA with only 17.3 ± 8.4 of spermatozoa remaining acrosome intact (P < 0.01) at this concentration. Sperm attachment to the plasmalemma was not related to penetration rates or induction of the acrosome reaction. Following capacitation of spermatozoa with 0.5 μM ionophore (-BSA) and fertilization of zona-free oocytes, we obtained a hamster-porcine metaphase plate yield of 13.9 ± 4.2 at 22-25 h post-insemination. A high percentage of spreads exhibited arrested sperm heads (48.7 ± 14.7). These results demonstrate that porcine sperm chromosome metaphases may be obtained following capacitation of spermatozoa and penetration of zona-free oocytes in vitro.

AB - Porcine spermatozoa were assessed for their ability to undergo the acrosome reaction and penetrate zona-free hamster eggs following treatment with ionophore, in order to select an optimal capacitation regime prior to fertilization and culture for sperm chromosomes. Penetration rates achieved 86.5% ± 6.0 at 0.5 μM ionophore -BSA with only 17.3 ± 8.4 of spermatozoa remaining acrosome intact (P < 0.01) at this concentration. Sperm attachment to the plasmalemma was not related to penetration rates or induction of the acrosome reaction. Following capacitation of spermatozoa with 0.5 μM ionophore (-BSA) and fertilization of zona-free oocytes, we obtained a hamster-porcine metaphase plate yield of 13.9 ± 4.2 at 22-25 h post-insemination. A high percentage of spreads exhibited arrested sperm heads (48.7 ± 14.7). These results demonstrate that porcine sperm chromosome metaphases may be obtained following capacitation of spermatozoa and penetration of zona-free oocytes in vitro.

UR - https://www.scopus.com/pages/publications/45249126444

U2 - 10.1016/0378-4320(89)90123-1

DO - 10.1016/0378-4320(89)90123-1

M3 - Article

AN - SCOPUS:45249126444

SN - 0378-4320

VL - 21

SP - 125

EP - 140

JO - Animal Reproduction Science

JF - Animal Reproduction Science

IS - 2

ER -

Fertilization of zona-free hamster ova by ionophore-treated boar sperm and the condensation of sperm chromosomes

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