Abstract
Summary A comparison of the 35S‐methionine metabolically labelled immunoreactive glycoproteins of immature and mature F. hepatica was carried out by one‐and two‐dimensional sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Sera of rabbits infected for 3 weeks reacted much more strongly with glycoproteins of immature flukes than with glycoproteins of mature flukes as compared to sera of rabbits infected for 9 weeks. Several of the immunoreactive glycoproteins were also released by immature F. hepatica into the culture medium. At least one was a component of the T1 type granules. Analysis of the in vitro translation products of mature F. hepatica indicated that the initial humoral immune response of rabbit hosts may be directed against carbohydrate moieties.
| Original language | English |
|---|---|
| Pages (from-to) | 643-657 |
| Number of pages | 15 |
| Journal | Parasite Immunology |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 1985 |
| Externally published | Yes |
Keywords
- Fasciola hepatica
- immature and mature flukes
- immunoprecipitation
- immunoreactive glycoproteins
- in vitro translation
- metabolic labelling
- polyacrylamide gel electrophoresis