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Extraction and Detection of Arabinogalactan Proteins

  • Zoë A. Popper

Research output: Chapter in Book or Conference Publication/ProceedingChapterpeer-review

16 Citations (Scopus)

Abstract

Arabinogalactan proteins are a diverse group of plant cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localise, and quantify them is fundamentally important. This chapter describes two commonly used methods, histological staining and radial gel diffusion, both of which utilise the ability of Yariv reagent to bind to AGPs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages245-254
Number of pages10
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume715
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Arabinogalactan protein detection
  • Arabinogalactan proteins
  • Radial gel diffusion
  • Semi-quantitative detection
  • Yariv reagent

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