Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in Human stool specimens

E. Collins; M. Glennon; S. Hanley; A. M. Murray; M. Cormican; T. Smith; M. Maher

doi:10.1128/JCM.39.11.4163-4165.2001

Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in Human stool specimens

E. Collins
, M. Glennon
, S. Hanley
, A. M. Murray
, M. Cormican
, T. Smith
, M. Maher

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

15 Citations (Scopus)

Abstract

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCP/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

Original language	English
Pages (from-to)	4163-4165
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	39
Issue number	11
DOIs	https://doi.org/10.1128/JCM.39.11.4163-4165.2001
Publication status	Published - 2001

Access to Document

10.1128/JCM.39.11.4163-4165.2001

Cite this

@article{8537cfa26a884ff3ae872d24a06b1517,

title = "Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in Human stool specimens",

abstract = "DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCP/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.",

author = "E. Collins and M. Glennon and S. Hanley and Murray, \{A. M.\} and M. Cormican and T. Smith and M. Maher",

year = "2001",

doi = "10.1128/JCM.39.11.4163-4165.2001",

language = "English",

volume = "39",

pages = "4163--4165",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in Human stool specimens

AU - Collins, E.

AU - Glennon, M.

AU - Hanley, S.

AU - Murray, A. M.

AU - Cormican, M.

AU - Smith, T.

AU - Maher, M.

PY - 2001

Y1 - 2001

N2 - DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCP/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

AB - DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCP/DNA probe positive for C. jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.

UR - https://www.scopus.com/pages/publications/0034764402

U2 - 10.1128/JCM.39.11.4163-4165.2001

DO - 10.1128/JCM.39.11.4163-4165.2001

M3 - Article

SN - 0095-1137

VL - 39

SP - 4163

EP - 4165

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in Human stool specimens

Abstract

Access to Document

Other files and links

Fingerprint

Cite this