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Evaluation of a PCR DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens

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Abstract

DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA probes specific for the Campylobacter genus, Campylobacter jejuni, and Campylobacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-positive specimens were PCR DNA probe positive for C jejuni. The assay is rapid and simple and can be applied to stool specimens for the detection of Campylobacter.
Original languageEnglish (Ireland)
Number of pages2
JournalJournal Of Clinical Microbiology
Volume39
Publication statusPublished - 1 Nov 2001

Authors (Note for portal: view the doc link for the full list of authors)

  • Authors
  • Collins, E;Glennon, M;Hanley, S;Murray, AM;Cormican, M;Smith, T;Maher, M

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