Evaluation of a novel PCR based diagnostic assay for the detection of Mycobacterium tuberculosis in sputum specimens

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Abstract

We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.
Original languageEnglish (Ireland)
Number of pages2
JournalJournal Of Clinical Microbiology
Volume34
Issue number9
DOIs
Publication statusPublished - 1 Jul 1996

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Authors (Note for portal: view the doc link for the full list of authors)

  • Authors
  • Maher, M., Glennon, M., Martinazzo, E., Turchetti, E., Marcolini, S., Smith, T.J. and Dawson, M.T.

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