Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples

Majella Maher; Maura Glennon; Giorgio Martinazzo; Elisabetta Turchetti; Stanislavo Marcolini; Terry Smith; Michael T. Dawson

doi:10.1128/jcm.34.9.2307-2308.1996

Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples

Majella Maher
, Maura Glennon
, Giorgio Martinazzo
, Elisabetta Turchetti
, Stanislavo Marcolini
, Terry Smith
, Michael T. Dawson

Infectious Disease

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

8 Citations (Scopus)

Abstract

We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.

Original language	English
Pages (from-to)	2307-2308
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	34
Issue number	9
DOIs	https://doi.org/10.1128/jcm.34.9.2307-2308.1996
Publication status	Published - Sep 1996

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SDG 3 Good Health and Well-being

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10.1128/jcm.34.9.2307-2308.1996

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abstract = "We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.",

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AU - Maher, Majella

AU - Glennon, Maura

AU - Martinazzo, Giorgio

AU - Turchetti, Elisabetta

AU - Marcolini, Stanislavo

AU - Smith, Terry

AU - Dawson, Michael T.

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AB - We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.

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ER -

Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples

Abstract

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