Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis

Babu R. Prasad; Enda O'Connell; Terry J. Smith; Valérie A. Gérard; Yurii K. Gun'Ko; Yury Rochev

doi:10.1007/978-1-61779-953-2_12

Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis

Babu R. Prasad
, Enda O'Connell
, Terry J. Smith
, Valérie A. Gérard
, Yurii K. Gun'Ko
, Yury Rochev

University of Galway
Trinity College Dublin

Research output: Chapter in Book or Conference Publication/Proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.

Original language	English
Title of host publication	Nanoparticles in Biology and Medicine
Subtitle of host publication	Methods and Protocols
Editors	Mikhail Soloviev
Publisher	Methods in Molecular Biology
Pages	171-183
Number of pages	13
ISBN (Print)	9781617799525
DOIs	https://doi.org/10.1007/978-1-61779-953-2_12
Publication status	Published - 1 Jan 2012

Publication series

Name	Methods in Molecular Biology
Volume	906
ISSN (Print)	1064-3745

Keywords

Gene expression
HPMEC cells
Quantum dots
RNA microarray

Authors (Note for portal: view the doc link for the full list of authors)

Authors
Prasad BR, O'Connell E, Smith TJ, GÃ©rard VA, Gun'ko YK, Rochev Y

Access to Document

10.1007/978-1-61779-953-2_12

Cite this

Prasad, B. R., O'Connell, E., Smith, T. J., Gérard, V. A., Gun'Ko, Y. K., & Rochev, Y. (2012). Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis. In M. Soloviev (Ed.), Nanoparticles in Biology and Medicine: Methods and Protocols (pp. 171-183). (Methods in Molecular Biology; Vol. 906). Methods in Molecular Biology. https://doi.org/10.1007/978-1-61779-953-2_12

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abstract = "Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.",

keywords = "Gene expression, HPMEC cells, Quantum dots, RNA microarray",

author = "Prasad, \{Babu R.\} and Enda O'Connell and Smith, \{Terry J.\} and G{\'e}rard, \{Val{\'e}rie A.\} and Gun'Ko, \{Yurii K.\} and Yury Rochev",

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language = "English",

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series = "Methods in Molecular Biology",

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}

Prasad, BR, O'Connell, E , Smith, TJ, Gérard, VA, Gun'Ko, YK & Rochev, Y 2012, Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis. in M Soloviev (ed.), Nanoparticles in Biology and Medicine: Methods and Protocols. Methods in Molecular Biology, vol. 906, Methods in Molecular Biology, pp. 171-183. https://doi.org/10.1007/978-1-61779-953-2_12

Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis. / Prasad, Babu R.; O'Connell, Enda ; Smith, Terry J. et al.
Nanoparticles in Biology and Medicine: Methods and Protocols. ed. / Mikhail Soloviev. Methods in Molecular Biology, 2012. p. 171-183 (Methods in Molecular Biology; Vol. 906).

Research output: Chapter in Book or Conference Publication/Proceeding › Chapter › peer-review

TY - CHAP

T1 - Evaluating the potential of quantum dots for in vitro biological studies

T2 - Effects on gene expression using microarray analysis

AU - Prasad, Babu R.

AU - O'Connell, Enda

AU - Smith, Terry J.

AU - Gérard, Valérie A.

AU - Gun'Ko, Yurii K.

AU - Rochev, Yury

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.

AB - Quantum dots have potential applications in the biomedical field and especially in bioimaging owing to their tunable fluorescent properties. Although many phenotypic studies have been carried out using QDs on different cell lines, only very few of them involved the analysis of the effect of QDs on gene expression. Here, we describe the application of microarray gene expression analysis for studying the differential expression of genes in the cells treated with QDs.

KW - Gene expression

KW - HPMEC cells

KW - Quantum dots

KW - RNA microarray

UR - https://www.scopus.com/pages/publications/84864829739

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DO - 10.1007/978-1-61779-953-2_12

M3 - Chapter

SN - 9781617799525

T3 - Methods in Molecular Biology

SP - 171

EP - 183

BT - Nanoparticles in Biology and Medicine

A2 - Soloviev, Mikhail

PB - Methods in Molecular Biology

ER -

Prasad BR, O'Connell E , Smith TJ, Gérard VA, Gun'Ko YK, Rochev Y. Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis. In Soloviev M, editor, Nanoparticles in Biology and Medicine: Methods and Protocols. Methods in Molecular Biology. 2012. p. 171-183. (Methods in Molecular Biology). doi: 10.1007/978-1-61779-953-2_12

Evaluating the potential of quantum dots for in vitro biological studies: Effects on gene expression using microarray analysis

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