TY - JOUR
T1 - Epidemiological cut-off values for Yersina ruckeri disc diffusion data generated by a standardised method
AU - Smith, Peter
AU - Le Devendec, Laëtitia
AU - Jouy, Eric
AU - Larvor, Emeline
AU - Verner-Jeffreys, David
AU - Joseph, Andrew Wokorac
AU - Stanton, Elliot
AU - Light, Edel
AU - Cortinovis, Luana
AU - Pretto, Tobia
AU - Manfrin, Amedeo
AU - Boitard, Pierre Marie
AU - Jamin, Matthieu
AU - Keck, Nicolas
AU - Le Breton, Alain
AU - Thuillier, Benoit
AU - Ravaille, Christian
AU - Baron, Sandrine
N1 - Publisher Copyright:
© The authors 2024.
PY - 2024/4/25
Y1 - 2024/4/25
N2 - In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this ana - lysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.
AB - In order to establish the meaning of data generated in antimicrobial agent susceptibility tests, it is necessary to develop internationally harmonised interpretive criteria. Currently, such criteria have not been developed for data generated in studies of the susceptibility of the fish pathogen Yersinia ruckeri. This work generated the data that would be required to set epidemiological cut-off values for the susceptibility data of this species that had been generated using a standardised disc diffusion method that specified the use of Mueller Hinton agar and incubation at 22°C for 24-28 h. Using this method, sets of inhibition zones data for 4 antimicrobial agents were generated by 3 independent laboratories. The data from these laboratories were aggregated and analysed using the statistically based normalised resistance interpretation. For ampicillin, florfenicol, oxytetracycline and trimethoprim-sulfamethoxazole the cut-off values calculated by this ana - lysis were ≥16, ≥23, ≥24 and ≥30 mm, respectively. Evidence is presented demonstrating that the data for these 4 agents was of sufficient quantity and quality that they could be used by the relevant authorities to set internationally harmonised, consensus epidemiological cut-off values for Y. ruckeri.
KW - Antimicrobial susceptibility
KW - CLSI VET04
KW - Disc diffusion
KW - Normalized resistance interpretation
KW - Yersinia ruckeri
UR - http://www.scopus.com/inward/record.url?scp=85191408927&partnerID=8YFLogxK
U2 - 10.3354/dao03779
DO - 10.3354/dao03779
M3 - Article
SN - 0177-5103
VL - 158
SP - 21
EP - 25
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
ER -