Abstract
We describe a modification of the most probable number (MPN) method for rapid enumeration of antimicrobial-resistant E. coli in aqueous environmental samples. E. coli (total and antimicrobial-resistant) were enumerated in effluent samples from a hospital (n = 17), municipal sewer upstream (n = 5) and downstream (n = 5) from the hospital, effluent from throughout the treatment process (n = 4) and treated effluent samples (n = 13). Effluent downstream from hospital contained a higher proportion of antimicrobial-resistant E. coli compared with upstream. Waste water treatment reduced numbers of E. coli (total and antimicrobial-resistant) however antimicrobial-resistant E. coli were not eliminated and E. coli resistant to cefotaxime (including extended-spectrum beta-lactamase (ESBL) producers), ciprofloxacin and cefoxitin were present in treated effluent.We describe a modification of the most probable number (MPN) method for rapid enumeration of antimicrobial-resistant E. coli in aqueous environmental samples. E. coli (total and antimicrobial-resistant) were enumerated in effluent samples from a hospital (n = 17), municipal sewer upstream (n = 5) and downstream (n = 5) from the hospital, effluent from throughout the treatment process (n = 4) and treated effluent samples (n = 13). Effluent downstream from hospital contained a higher proportion of antimicrobial-resistant E. coli compared with upstream. Waste water treatment reduced numbers of E. coli (total and antimicrobial-resistant) however antimicrobial-resistant E. coli were not eliminated and E. coli resistant to cefotaxime (including extended-spectrum beta-lactamase (ESBL) producers), ciprofloxacin and cefoxitin were present in treated effluent.
| Original language | English (Ireland) |
|---|---|
| Journal | Appl Environ Microbiol |
| Publication status | Published - 1 Jun 2010 |
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Galvin, S.,Boyle, F.,Hickey, P.,Vellinga, A.,Morris, D.,Cormican, M.