TY - JOUR
T1 - Engineering in vitro microenvironments for cell based therapies and drug discovery
AU - Cigognini, Daniela
AU - Lomas, Alexander
AU - Kumar, Pramod
AU - Satyam, Abhigyan
AU - English, Andrew
AU - Azeem, Ayesha
AU - Pandit, Abhay
AU - Zeugolis, Dimitrios
PY - 2013/11
Y1 - 2013/11
N2 - Traditional ex vivo culture setups fail to imitate the native tissue niche, leading to cellular senescence, phenotypic drift, growth arrest and loss of stem cell multipotency. Growing evidence suggests that surface topography, substrate stiffness, mechanical stimulation, oxygen tension and localised density influence cellular functions and longevity, enhance tissue-specific extracellular matrix deposition and direct stem cell differentiation. In this review, we discuss how these cues will facilitate engineering of physiological in vitro microenvironments to enable clinical translation of cell based therapies and development of in vitro models for drug discovery applications.
AB - Traditional ex vivo culture setups fail to imitate the native tissue niche, leading to cellular senescence, phenotypic drift, growth arrest and loss of stem cell multipotency. Growing evidence suggests that surface topography, substrate stiffness, mechanical stimulation, oxygen tension and localised density influence cellular functions and longevity, enhance tissue-specific extracellular matrix deposition and direct stem cell differentiation. In this review, we discuss how these cues will facilitate engineering of physiological in vitro microenvironments to enable clinical translation of cell based therapies and development of in vitro models for drug discovery applications.
UR - https://www.scopus.com/pages/publications/84886952145
U2 - 10.1016/j.drudis.2013.06.007
DO - 10.1016/j.drudis.2013.06.007
M3 - Review article
SN - 1359-6446
VL - 18
SP - 1099
EP - 1108
JO - Drug Discovery Today
JF - Drug Discovery Today
IS - 21-22
ER -