Engineered assembly of a protein-cucurbituril biohybrid

Francesca Guagnini; Sylvain Engilberge; Kiefer O. Ramberg; Javier Pérez; Peter B. Crowley

doi:10.1039/c9cc07198a

Engineered assembly of a protein-cucurbituril biohybrid

Francesca Guagnini, Sylvain Engilberge, Kiefer O. Ramberg, Javier Pérez, Peter B. Crowley

Cellular & Molecular Medicine

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

27 Citations (Scopus)

Abstract

A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.

Original language	English
Pages (from-to)	360-363
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	3
DOIs	https://doi.org/10.1039/c9cc07198a
Publication status	Published - 2020

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10.1039/c9cc07198a

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title = "Engineered assembly of a protein-cucurbituril biohybrid",

abstract = "A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.",

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AU - Guagnini, Francesca

AU - Engilberge, Sylvain

AU - Ramberg, Kiefer O.

AU - Pérez, Javier

AU - Crowley, Peter B.

N1 - Publisher Copyright: This journal is © The Royal Society of Chemistry.

PY - 2020

Y1 - 2020

N2 - A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.

AB - A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.

UR - https://www.scopus.com/pages/publications/85077402392

U2 - 10.1039/c9cc07198a

DO - 10.1039/c9cc07198a

M3 - Article

C2 - 31825399

AN - SCOPUS:85077402392

SN - 1359-7345

VL - 56

SP - 360

EP - 363

JO - Chemical Communications

JF - Chemical Communications

IS - 3

ER -

Engineered assembly of a protein-cucurbituril biohybrid

Abstract

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