Electrically ‘wired’ tyrosinase enzyme inhibition electrode for the detection of respiratory poisons

Gemma Robinson; Dónal Leech; Malcolm R. Smyth

doi:10.1002/elan.1140071008

Electrically ‘wired’ tyrosinase enzyme inhibition electrode for the detection of respiratory poisons

Gemma Robinson, Dónal Leech, Malcolm R. Smyth

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

30 Citations (Scopus)

Abstract

The use of the solution redox species, [Os(bpy)₂Cl₂]^+/0, [Os(bpy)₂(MeIm)Cl]^2+/+ and [Fe(CN)₆]^4−/3−, where bpy is 2,2‐bipyridine and MeIm is N‐methylimidazole, as electron mediators in the enzymatic reduction of oxygen by tyrosinase is investigated. Co‐immobilization of both enzyme and an osmium redox mediator in a hydrogel on glassy carbon electrodes results in a biosensor for the ‘reagentless’ addressing of enzyme activity, consuming only oxygen present in solution. Immobilized enzyme inhibition biosensors can thus be constructed for the detection of tyrosinase inhibitors, such as sodium azide, using this approach. The enzyme inhibition biosensor can detect levels of azide as low as 5 × 10⁻⁶ mol dm⁻³ in solution and may be useful in environmental monitoring applications and as an early warning poison sensor.

Original language	English
Pages (from-to)	952-957
Number of pages	6
Journal	Electroanalysis
Volume	7
Issue number	10
DOIs	https://doi.org/10.1002/elan.1140071008
Publication status	Published - Oct 1995
Externally published	Yes

Keywords

Biosensor
Enzyme Inhibiton Electrode
Mediators
Reagentless
Tyrosinase

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10.1002/elan.1140071008

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@article{549940576f6a4d4e9521486c15b14527,

title = "Electrically {\textquoteleft}wired{\textquoteright} tyrosinase enzyme inhibition electrode for the detection of respiratory poisons",

abstract = "The use of the solution redox species, [Os(bpy)2Cl2]+/0, [Os(bpy)2(MeIm)Cl]2+/+ and [Fe(CN)6]4−/3−, where bpy is 2,2‐bipyridine and MeIm is N‐methylimidazole, as electron mediators in the enzymatic reduction of oxygen by tyrosinase is investigated. Co‐immobilization of both enzyme and an osmium redox mediator in a hydrogel on glassy carbon electrodes results in a biosensor for the {\textquoteleft}reagentless{\textquoteright} addressing of enzyme activity, consuming only oxygen present in solution. Immobilized enzyme inhibition biosensors can thus be constructed for the detection of tyrosinase inhibitors, such as sodium azide, using this approach. The enzyme inhibition biosensor can detect levels of azide as low as 5 × 10−6 mol dm−3 in solution and may be useful in environmental monitoring applications and as an early warning poison sensor.",

keywords = "Biosensor, Enzyme Inhibiton Electrode, Mediators, Reagentless, Tyrosinase",

author = "Gemma Robinson and D{\'o}nal Leech and Smyth, \{Malcolm R.\}",

year = "1995",

month = oct,

doi = "10.1002/elan.1140071008",

language = "English",

volume = "7",

pages = "952--957",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley-VCH Verlag",

number = "10",

}

TY - JOUR

T1 - Electrically ‘wired’ tyrosinase enzyme inhibition electrode for the detection of respiratory poisons

AU - Robinson, Gemma

AU - Leech, Dónal

AU - Smyth, Malcolm R.

PY - 1995/10

Y1 - 1995/10

N2 - The use of the solution redox species, [Os(bpy)2Cl2]+/0, [Os(bpy)2(MeIm)Cl]2+/+ and [Fe(CN)6]4−/3−, where bpy is 2,2‐bipyridine and MeIm is N‐methylimidazole, as electron mediators in the enzymatic reduction of oxygen by tyrosinase is investigated. Co‐immobilization of both enzyme and an osmium redox mediator in a hydrogel on glassy carbon electrodes results in a biosensor for the ‘reagentless’ addressing of enzyme activity, consuming only oxygen present in solution. Immobilized enzyme inhibition biosensors can thus be constructed for the detection of tyrosinase inhibitors, such as sodium azide, using this approach. The enzyme inhibition biosensor can detect levels of azide as low as 5 × 10−6 mol dm−3 in solution and may be useful in environmental monitoring applications and as an early warning poison sensor.

AB - The use of the solution redox species, [Os(bpy)2Cl2]+/0, [Os(bpy)2(MeIm)Cl]2+/+ and [Fe(CN)6]4−/3−, where bpy is 2,2‐bipyridine and MeIm is N‐methylimidazole, as electron mediators in the enzymatic reduction of oxygen by tyrosinase is investigated. Co‐immobilization of both enzyme and an osmium redox mediator in a hydrogel on glassy carbon electrodes results in a biosensor for the ‘reagentless’ addressing of enzyme activity, consuming only oxygen present in solution. Immobilized enzyme inhibition biosensors can thus be constructed for the detection of tyrosinase inhibitors, such as sodium azide, using this approach. The enzyme inhibition biosensor can detect levels of azide as low as 5 × 10−6 mol dm−3 in solution and may be useful in environmental monitoring applications and as an early warning poison sensor.

KW - Biosensor

KW - Enzyme Inhibiton Electrode

KW - Mediators

KW - Reagentless

KW - Tyrosinase

UR - https://www.scopus.com/pages/publications/84987460223

U2 - 10.1002/elan.1140071008

DO - 10.1002/elan.1140071008

M3 - Article

AN - SCOPUS:84987460223

SN - 1040-0397

VL - 7

SP - 952

EP - 957

JO - Electroanalysis

JF - Electroanalysis

IS - 10

ER -

Electrically ‘wired’ tyrosinase enzyme inhibition electrode for the detection of respiratory poisons

Abstract

Keywords

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