Effects of Polydopamine Functionalization on Boron Nitride Nanotube Dispersion and Cytocompatibility

Manus Biggs; Abhay Shashikant Pandit; Alanna Stanley

doi:10.1021/acs.bioconjchem.5b00257

Effects of Polydopamine Functionalization on Boron Nitride Nanotube Dispersion and Cytocompatibility

University of Galway
University of Basque
University of Limerick

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

43 Citations (Scopus)

Abstract

Boron nitride nanotubes (BNNTs) have unique physical properties, of value in biomedical applications; however, their dispersion and functionalization represent a critical challenge in their successful employment as biomaterials. In the present study, we report a process for the efficient disentanglement of BNNTs via a dual surfactant polydopamine (PD) process. High-resolution transmission electron microscopy (HR-TEM) indicated that individual BNNTs become coated with a uniform PD nanocoating, which significantly enhanced dispersion of BNNTs in aqueous solutions. Furthermore, the cytocompatibility of PD-coated BNNTs was assessed in vitro with cultured human osteoblasts (HOBs) at concentrations of 1, 10, and 30 mu g mL and over three time-points (24, 48, and 72 h). In this study it was demonstrated that PD-functionalized BNNTs become individually localized within the cytoplasm by endosomal escape and that concentrations of up to 30 mu g mL of PD-BNNTs were cytocompatible in HOBs cells following 72 h of exposure.

Original language	English (Ireland)
Pages (from-to)	2025-2037
Number of pages	13
Journal	Bioconjugate Chemistry
Volume	26
Issue number	10
DOIs	https://doi.org/10.1021/acs.bioconjchem.5b00257
Publication status	Published - 1 Jan 2015

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Authors
Marc A. Fernandez-Yague, Aitor Larrañaga,Olga Gladkovskaya,Alanna Stanley,Ghazal Tadayyon,Yina Guo, Jose-Ramon Sarasua, Syed A. M. Tofail, Dimitrios I. Zeugolis, Abhay Pandit, Manus J. Biggs
Fernandez-Yague, MA,Larranaga, A,Gladkovskaya, O,Stanley, A,Tadayyon, G,Guo, YN,Sarasua, JR,Tofail, SAM,Zeugolis, DI,Pandit, A,Biggs, MJ

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title = "Effects of Polydopamine Functionalization on Boron Nitride Nanotube Dispersion and Cytocompatibility",

abstract = "Boron nitride nanotubes (BNNTs) have unique physical properties, of value in biomedical applications; however, their dispersion and functionalization represent a critical challenge in their successful employment as biomaterials. In the present study, we report a process for the efficient disentanglement of BNNTs via a dual surfactant polydopamine (PD) process. High-resolution transmission electron microscopy (HR-TEM) indicated that individual BNNTs become coated with a uniform PD nanocoating, which significantly enhanced dispersion of BNNTs in aqueous solutions. Furthermore, the cytocompatibility of PD-coated BNNTs was assessed in vitro with cultured human osteoblasts (HOBs) at concentrations of 1, 10, and 30 mu g mL and over three time-points (24, 48, and 72 h). In this study it was demonstrated that PD-functionalized BNNTs become individually localized within the cytoplasm by endosomal escape and that concentrations of up to 30 mu g mL of PD-BNNTs were cytocompatible in HOBs cells following 72 h of exposure.",

author = "Manus Biggs and Pandit, \{Abhay Shashikant\} and Alanna Stanley",

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AU - Biggs, Manus

AU - Pandit, Abhay Shashikant

AU - Stanley, Alanna

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Boron nitride nanotubes (BNNTs) have unique physical properties, of value in biomedical applications; however, their dispersion and functionalization represent a critical challenge in their successful employment as biomaterials. In the present study, we report a process for the efficient disentanglement of BNNTs via a dual surfactant polydopamine (PD) process. High-resolution transmission electron microscopy (HR-TEM) indicated that individual BNNTs become coated with a uniform PD nanocoating, which significantly enhanced dispersion of BNNTs in aqueous solutions. Furthermore, the cytocompatibility of PD-coated BNNTs was assessed in vitro with cultured human osteoblasts (HOBs) at concentrations of 1, 10, and 30 mu g mL and over three time-points (24, 48, and 72 h). In this study it was demonstrated that PD-functionalized BNNTs become individually localized within the cytoplasm by endosomal escape and that concentrations of up to 30 mu g mL of PD-BNNTs were cytocompatible in HOBs cells following 72 h of exposure.

AB - Boron nitride nanotubes (BNNTs) have unique physical properties, of value in biomedical applications; however, their dispersion and functionalization represent a critical challenge in their successful employment as biomaterials. In the present study, we report a process for the efficient disentanglement of BNNTs via a dual surfactant polydopamine (PD) process. High-resolution transmission electron microscopy (HR-TEM) indicated that individual BNNTs become coated with a uniform PD nanocoating, which significantly enhanced dispersion of BNNTs in aqueous solutions. Furthermore, the cytocompatibility of PD-coated BNNTs was assessed in vitro with cultured human osteoblasts (HOBs) at concentrations of 1, 10, and 30 mu g mL and over three time-points (24, 48, and 72 h). In this study it was demonstrated that PD-functionalized BNNTs become individually localized within the cytoplasm by endosomal escape and that concentrations of up to 30 mu g mL of PD-BNNTs were cytocompatible in HOBs cells following 72 h of exposure.

UR - https://www.scopus.com/pages/publications/84945560709

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DO - 10.1021/acs.bioconjchem.5b00257

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JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 10

ER -

Effects of Polydopamine Functionalization on Boron Nitride Nanotube Dispersion and Cytocompatibility

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