Effect of hepatic nerve stimulation and norepinephrine on the laser doppler flux signal from the surface of the perfused rat liver

The effect of hepatic nerve stimulation and norepinephrine (NE) on the laser Doppler signal from the surface of the perfused rat liver was tested. The livers from male Wistar rats were perfused in situ via the portal vein with Krebs-Henseleit buffer containing 20% bovine erythrocytes (37 °C, pH 7.4) and total liver blood flow (TLBF) was by timed collection of effluent. Portal vascular resistance (PVR) was calculated from the pressure difference across the liver. Linearity of laser Doppler flowmetry (LDF) with TLBF was confirmed in all preparations. Stimulation of the hepatic nerves (2 ms, 20 V) was performed at frequencies between 0.5 and 20 Hz (n = 11). NE was added to the buffer at concentrations between 10^-10 and 10^-6M(n = 8). A stimulus-dependent rise in PVR occurred during hepatic nerve stimulation (basal, 3.11 ± 0.26 dyn s cm^-5) and NE administration (basal, 2.62 ± 0.29 dyn s cm^-5), with a maximum effect at 20 Hz (311 ± 45%) and 10^-6M(591 ± 72%), respectively. Both LDF and TLBF fell during nerve stimulation and NE. A linear relationship (r = 0.99; p < 0.001) between change in TLBF (%) and LDF flux (%) was found for NE (10^-10 to 10^-6M). During nerve stimulation, the fall in TLBF and LDF flux was linear with the logarithm of stimulus frequency and reached a maximum at 10 and 20 Hz, respectively. At a stimulus frequency of 20 Hz, the change in LDF was significantly different from the change in TLBF (p < 0.001). We conclude from our findings that during high-frequency hepatic nerve stimulation, LDF underestimates TLBF.