Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures

Jeroen Charité; Wim de Graaff; Sanbing Shen; Jacqueline Deschamps

doi:10.1016/0092-8674(94)90524-X

Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures

Jeroen Charité, Wim de Graaff, Sanbing Shen, Jacqueline Deschamps

Hubrecht Laboratory, Netherlands Institute for Developmental Biology

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

227 Citations (Scopus)

Abstract

Transgenic embryos were generated carrying a Hoxb-8 transgene under control of the mouse RARβ2 promoter, which extends the normal expression domain to more anterior regions of the embryo. These embryos showed mirror-image duplications in the forelimb, analogous to the duplications observed in chick in response to transplantation of a ZPA to the anterior margin of the limb bud. Examination of Sonic hedgehog, Fgf-4, and Hoxd-11 gene expression confirmed that a second ZPA had been generated at the anterior side of the limb bud. Besides other alterations, posterior homeotic transformations of axial structures were observed, involving the first spinal (Frorlep's) ganglion and several cervical vertebrae.

Original language	English
Pages (from-to)	589-601
Number of pages	13
Journal	Cell
Volume	78
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(94)90524-X
Publication status	Published - 26 Aug 1994
Externally published	Yes

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title = "Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures",

abstract = "Transgenic embryos were generated carrying a Hoxb-8 transgene under control of the mouse RARβ2 promoter, which extends the normal expression domain to more anterior regions of the embryo. These embryos showed mirror-image duplications in the forelimb, analogous to the duplications observed in chick in response to transplantation of a ZPA to the anterior margin of the limb bud. Examination of Sonic hedgehog, Fgf-4, and Hoxd-11 gene expression confirmed that a second ZPA had been generated at the anterior side of the limb bud. Besides other alterations, posterior homeotic transformations of axial structures were observed, involving the first spinal (Frorlep's) ganglion and several cervical vertebrae.",

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AU - Charité, Jeroen

AU - de Graaff, Wim

AU - Shen, Sanbing

AU - Deschamps, Jacqueline

PY - 1994/8/26

Y1 - 1994/8/26

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AB - Transgenic embryos were generated carrying a Hoxb-8 transgene under control of the mouse RARβ2 promoter, which extends the normal expression domain to more anterior regions of the embryo. These embryos showed mirror-image duplications in the forelimb, analogous to the duplications observed in chick in response to transplantation of a ZPA to the anterior margin of the limb bud. Examination of Sonic hedgehog, Fgf-4, and Hoxd-11 gene expression confirmed that a second ZPA had been generated at the anterior side of the limb bud. Besides other alterations, posterior homeotic transformations of axial structures were observed, involving the first spinal (Frorlep's) ganglion and several cervical vertebrae.

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JO - Cell

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Ectopic expression of Hoxb-8 causes duplication of the ZPA in the forelimb and homeotic transformation of axial structures

Abstract

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