DNA probe for Aeromonas salmonicida

M. Hiney; M. T. Dawson; D. M. Heery; P. R. Smith; F. Gannon; R. Powell

doi:10.1128/aem.58.3.1039-1042.1992

DNA probe for Aeromonas salmonicida

M. Hiney, M. T. Dawson, D. M. Heery, P. R. Smith, F. Gannon, R. Powell

Infectious Disease

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Comment/debate

85 Citations (Scopus)

Abstract

A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial genera. The sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two A. salmonicida cells.

Original language	English
Pages (from-to)	1039-1042
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	58
Issue number	3
DOIs	https://doi.org/10.1128/aem.58.3.1039-1042.1992
Publication status	Published - 1992

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10.1128/aem.58.3.1039-1042.1992

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title = "DNA probe for Aeromonas salmonicida",

abstract = "A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial genera. The sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two A. salmonicida cells.",

author = "M. Hiney and Dawson, {M. T.} and Heery, {D. M.} and Smith, {P. R.} and F. Gannon and R. Powell",

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AU - Hiney, M.

AU - Dawson, M. T.

AU - Heery, D. M.

AU - Smith, P. R.

AU - Gannon, F.

AU - Powell, R.

PY - 1992

Y1 - 1992

N2 - A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial genera. The sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two A. salmonicida cells.

AB - A DNA fragment that is specific to Aeromonas salmonicida has been isolated from a genomic DNA library by differential hybridization. The specificity of this fragment as a DNA probe for A. salmonicida was shown by hybridization against reference strains and clinical isolates of A. salmonicida, related aeromonads, and species from several other bacterial genera. The sensitivity of detection by a polymerase chain reaction test, based on this fragment, was approximately two A. salmonicida cells.

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U2 - 10.1128/aem.58.3.1039-1042.1992

DO - 10.1128/aem.58.3.1039-1042.1992

M3 - Comment/debate

SN - 0099-2240

VL - 58

SP - 1039

EP - 1042

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

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ER -

DNA probe for Aeromonas salmonicida

Abstract

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