DNA mismatch correction in a defined system

R. S. Lahue; K. G. Au; P. Modrich

doi:10.1126/science.2665076

DNA mismatch correction in a defined system

R. S. Lahue
, K. G. Au
, P. Modrich

Duke University Medical Center

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

482 Citations (Scopus)

Abstract

DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.

Original language	English
Pages (from-to)	160-164
Number of pages	5
Journal	Science
Volume	245
Issue number	4914
DOIs	https://doi.org/10.1126/science.2665076
Publication status	Published - 1989
Externally published	Yes

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abstract = "DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.",

author = "Lahue, \{R. S.\} and Au, \{K. G.\} and P. Modrich",

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AB - DNA mismatch correction is a strand-specific process involving recognition of noncomplementary Watson-Crick nucleotide pairs and participation of widely separated DNA sites. The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, exonuclease I, DNA ligase, along with ATP (adenosine triphosphate), and the four deoxynucleoside triphosphates. This set of proteins can process seven of the eight base-base mismatches in a strand-specific reaction that is directed by the state of methylation of a single d(GATC) sequence located 1 kilobase from the mispair.

UR - https://www.scopus.com/pages/publications/0024396565

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M3 - Article

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EP - 164

JO - Science

JF - Science

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ER -

DNA mismatch correction in a defined system

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