TY - JOUR
T1 - Dissemination of extensively drug-resistant NDM-producing Providencia stuartii in Europe linked to patients transferred from Ukraine, March 2022 to March 2023
AU - Witteveen, Sandra
AU - Hans, Jörg B.
AU - Izdebski, Radosław
AU - Hasman, Henrik
AU - Samuelsen, Ørjan
AU - Dortet, Laurent
AU - Pfeifer, Yvonne
AU - Delappe, Niall
AU - Oteo-Iglesias, Jesús
AU - Żabicka, Dorota
AU - Cormican, Martin
AU - Sandfort, Mirco
AU - Reichert, Felix
AU - Pöntinen, Anna K.
AU - Fischer, Martin A.
AU - Verkaik, Nelianne
AU - Pérez-Vazquez, María
AU - Pfennigwerth, Niels
AU - Hammerum, Anette M.
AU - Hallstrøm, Søren
AU - Biedrzycka, Marta
AU - Räisänen, Kati
AU - Wielders, Cornelia C.H.
AU - Urbanowicz, Paweł
AU - de Haan, Angela
AU - Westmo, Karin
AU - Landman, Fabian
AU - van der Heide, Han G.J.
AU - Lansu, Simon
AU - Zwittink, Romy D.
AU - Notermans, Daan W.
AU - Guzek, Aneta
AU - Kondratiuk, Viacheslav
AU - Salmanov, Aidyn
AU - Haller, Sebastian
AU - Linkevicius, Marius
AU - Gatermann, Sören
AU - Kohlenberg, Anke
AU - Gniadkowski, Marek
AU - Werner, Guido
AU - Hendrickx, Antoni P.A.
N1 - Publisher Copyright:
© 2024 European Centre for Disease Prevention and Control (ECDC). All rights reserved.
PY - 2024/6/6
Y1 - 2024/6/6
N2 - Background: The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related to Ukrainian patients. Aim: To investigate the genomic epidemiology of New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from Ukrainian patients among European countries. Methods: Whole-genome sequencing of 66 isolates sampled in 2022–2023 in 10 European countries enabled whole-genome multilocus sequence typing (wgMLST), identification of resistance genes, replicons, and plasmid reconstructions. Five blaNDM-1-carrying-P. stuartii isolates underwent antimicrobial susceptibility testing (AST). Transferability to Escherichia coli of a blaNDM-1-carrying plasmid from a patient strain was assessed. Epidemiological characteristics of patients with NDM-producing P. stuartii were gathered by questionnaire. Results: wgMLST of the 66 isolates revealed two genetic clusters unrelated to Ukraine and three linked toUkrainian patients. Of these three, two comprised blaNDM-1-carrying-P. stuartii and the third blaNDM-5-carrying-P. stuartii. The blaNDM-1 clusters (PstCluster-001, n=22 isolates; PstCluster-002, n=8 isolates) comprised strains from seven and four countries, respectively. The blaNDM-5 cluster (PstCluster-003) included 13 isolates from six countries. PstCluster-001 and PstCluster-002 isolates carried an MDR plasmid harbouring blaNDM-1,blaOXA-10, blaCMY-16, rmtC and armA, which was transferrable in vitro and, for some Ukrainian patients, shared by other Enterobacterales. AST revealed PstCluster-001 isolates to be extensively drug-resistant (XDR), but susceptible to cefiderocol and aztreonam–avibactam. Patients with data on age (n=41) were 19–74years old; of 49 with information on sex, 38 were male. Conclusion: XDR P. stuartii were introduced into European countries, requiring increased awareness and precautions when treating patients from conflict-affected areas.
AB - Background: The war in Ukraine led to migration of Ukrainian people. Early 2022, several European national surveillance systems detected multidrug-resistant (MDR) bacteria related to Ukrainian patients. Aim: To investigate the genomic epidemiology of New Delhi metallo-β-lactamase (NDM)-producing Providencia stuartii from Ukrainian patients among European countries. Methods: Whole-genome sequencing of 66 isolates sampled in 2022–2023 in 10 European countries enabled whole-genome multilocus sequence typing (wgMLST), identification of resistance genes, replicons, and plasmid reconstructions. Five blaNDM-1-carrying-P. stuartii isolates underwent antimicrobial susceptibility testing (AST). Transferability to Escherichia coli of a blaNDM-1-carrying plasmid from a patient strain was assessed. Epidemiological characteristics of patients with NDM-producing P. stuartii were gathered by questionnaire. Results: wgMLST of the 66 isolates revealed two genetic clusters unrelated to Ukraine and three linked toUkrainian patients. Of these three, two comprised blaNDM-1-carrying-P. stuartii and the third blaNDM-5-carrying-P. stuartii. The blaNDM-1 clusters (PstCluster-001, n=22 isolates; PstCluster-002, n=8 isolates) comprised strains from seven and four countries, respectively. The blaNDM-5 cluster (PstCluster-003) included 13 isolates from six countries. PstCluster-001 and PstCluster-002 isolates carried an MDR plasmid harbouring blaNDM-1,blaOXA-10, blaCMY-16, rmtC and armA, which was transferrable in vitro and, for some Ukrainian patients, shared by other Enterobacterales. AST revealed PstCluster-001 isolates to be extensively drug-resistant (XDR), but susceptible to cefiderocol and aztreonam–avibactam. Patients with data on age (n=41) were 19–74years old; of 49 with information on sex, 38 were male. Conclusion: XDR P. stuartii were introduced into European countries, requiring increased awareness and precautions when treating patients from conflict-affected areas.
UR - http://www.scopus.com/inward/record.url?scp=85195438763&partnerID=8YFLogxK
U2 - 10.2807/1560-7917.ES.2024.29.23.2300616
DO - 10.2807/1560-7917.ES.2024.29.23.2300616
M3 - Article
C2 - 38847120
AN - SCOPUS:85195438763
SN - 1025-496X
VL - 9
JO - Eurosurveillance
JF - Eurosurveillance
IS - 23
M1 - pii=2300616
ER -