Abstract
We developed a direct microtiter plate enzyme immunoassay to measure estradiol-17 beta in saliva. The assay has a commercially available monoclonal antibody, raised against estradiol-17 beta-6-carboxymethyloxime-bovine serum albumin, and a homologous horseradish peroxidase conjugate measured colorimetrically. The detection limit (equivalent to B-0 - 3 SD) is 365 amol well or 7.3 pmoI L when 50-mu L, samples are assayed. Cross-reactivity with estrone and estriol, testosterone, or progesterone is 0.2%. Estradiol-17 beta was measured in daily samples over five natural menstrual cycles and eight cycles stimulated as a preliminary to in vitro fertilization, and the concentrations and fluctuations found agreed with previously published data. This method gives results in similar to 3 h and may be useful for fertility monitoring and management.
| Original language | English (Ireland) |
|---|---|
| Number of pages | 6 |
| Journal | Clinical Chemistry |
| Volume | 43 |
| Publication status | Published - 1 Jul 1997 |
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Tamate, K,Charleton, M,Gosling, JP,Egan, D,Ishikawa, M,Fottrell, PF,Kane, MM