Abstract
Replication protein A (RPA) is a stable heterotrimeric complex consisting of p70, p32 and p14 subunits. The protein plays a crucial role in SV40 minichromosome replication. Peptides of p70 representing interaction sites for the smaller two subunits, DNA as well as the viral initiator protein large T-antigen (Tag) and the cellular DNA polymerase a-primase (Pol) all interfered with the replication process indicating the importance of the different p70 activities in this process. Inhibition by the peptide disrupting protein-protein interactions was observed only during the pre-initiation stage prior to primer synthesis, suggesting the formation of a stable initiation complex between RPA, Tag and Pol at the primer end. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
| Original language | English (Ireland) |
|---|---|
| Pages (from-to) | 3973-3978 |
| Number of pages | 6 |
| Journal | Febs Letters |
| Volume | 581 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - 1 Aug 2007 |
Keywords
- Protein-protein interaction
- Replication protein A
- SV40 DNA replication
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Taneja, P,Boche, I,Hartmann, H,Nasheuer, HP,Grosse, F,Fanning, E,Weisshart, K
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