Determination of the nonnutritive sweetener sodium cyclopentylsulfamate and three of its metabolites, cyclopentylamine, cyclopentanone, and cyclopentanol, in urine of rats and rabbits

The nonnutritive sweetener sodium cyclopentylsulfamate was fed to Wistar albino rats and New Zealand White rabbits. Urine was collected for 3 days after feeding, combined, and examined for the metabolites cyclopentylamine, cyclopentanone, and cyclopentanol and for sodium cyclopentylsulfamate. A GLC method allowing the simultaneous analysis (with convenient retention times) for the amine, ketone, and alcoholic metabolites was developed. Sodium cyclopentylsulfamate was assayed by hydrolysis in acidified dioxane‐water and subsequent measurement of the absorbance of the product formed (λ_max = 490 nm) by the liberated amine with p‐benzoquinone. The average conversion to cyclopentylamine, cyclopentanone, and cyclopentanol was 0.103, 0.171, and 0.054% in the rabbit and 0.057, 0.016, and 0.008% in the rat, respectively.