Detection of anticentromere antibodies using recombinant human CENP-A protein

Dongxu Sun; Antigona Martinez; Kevin F. Sullivan; Gordon C. Sharp; Sallie O. Hoch

doi:10.1002/art.1780390520

Detection of anticentromere antibodies using recombinant human CENP-A protein

Dongxu Sun, Antigona Martinez, Kevin F. Sullivan, Gordon C. Sharp, Sallie O. Hoch

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

13 Citations (Scopus)

Abstract

Objective. To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by enzyme-linked immunosorbent assay (ELISA). Results. Of the ACA+ sera studied (n = 38), 95% were positive when tested against the rCENP-A in the ELISA system. Of the ACA- sera (n = 100), only 2% gave false-positive results in the assay. There was good correlation between the recombinant and bona fide antigens in assaying for ACA reactivity. Conclusion. CENP-A is a significant ACA target. The availability of the rCENP-A assay is a valuable adjunct to the previously described rCENP-B assay in analyses of the clinical significance of ACA.

Original language	English
Pages (from-to)	863-867
Number of pages	5
Journal	Arthritis and Rheumatism
Volume	39
Issue number	5
DOIs	https://doi.org/10.1002/art.1780390520
Publication status	Published - May 1996
Externally published	Yes

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title = "Detection of anticentromere antibodies using recombinant human CENP-A protein",

abstract = "Objective. To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by enzyme-linked immunosorbent assay (ELISA). Results. Of the ACA+ sera studied (n = 38), 95\% were positive when tested against the rCENP-A in the ELISA system. Of the ACA- sera (n = 100), only 2\% gave false-positive results in the assay. There was good correlation between the recombinant and bona fide antigens in assaying for ACA reactivity. Conclusion. CENP-A is a significant ACA target. The availability of the rCENP-A assay is a valuable adjunct to the previously described rCENP-B assay in analyses of the clinical significance of ACA.",

author = "Dongxu Sun and Antigona Martinez and Sullivan, \{Kevin F.\} and Sharp, \{Gordon C.\} and Hoch, \{Sallie O.\}",

year = "1996",

month = may,

doi = "10.1002/art.1780390520",

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volume = "39",

pages = "863--867",

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publisher = "John Wiley \& Sons Inc.",

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T1 - Detection of anticentromere antibodies using recombinant human CENP-A protein

AU - Sun, Dongxu

AU - Martinez, Antigona

AU - Sullivan, Kevin F.

AU - Sharp, Gordon C.

AU - Hoch, Sallie O.

PY - 1996/5

Y1 - 1996/5

N2 - Objective. To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by enzyme-linked immunosorbent assay (ELISA). Results. Of the ACA+ sera studied (n = 38), 95% were positive when tested against the rCENP-A in the ELISA system. Of the ACA- sera (n = 100), only 2% gave false-positive results in the assay. There was good correlation between the recombinant and bona fide antigens in assaying for ACA reactivity. Conclusion. CENP-A is a significant ACA target. The availability of the rCENP-A assay is a valuable adjunct to the previously described rCENP-B assay in analyses of the clinical significance of ACA.

AB - Objective. To evaluate CENP-A reactivity with anticentromere antibodies (ACA) using recombinant protein (rCENP-A). Methods. Human CENP-A antigen was overexpressed in insect cells using the baculovirus system. We tested for ACA activity against the full-length recombinant polypeptide by immunoblot and by enzyme-linked immunosorbent assay (ELISA). Results. Of the ACA+ sera studied (n = 38), 95% were positive when tested against the rCENP-A in the ELISA system. Of the ACA- sera (n = 100), only 2% gave false-positive results in the assay. There was good correlation between the recombinant and bona fide antigens in assaying for ACA reactivity. Conclusion. CENP-A is a significant ACA target. The availability of the rCENP-A assay is a valuable adjunct to the previously described rCENP-B assay in analyses of the clinical significance of ACA.

UR - https://www.scopus.com/pages/publications/0029924604

U2 - 10.1002/art.1780390520

DO - 10.1002/art.1780390520

M3 - Article

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AN - SCOPUS:0029924604

SN - 0004-3591

VL - 39

SP - 863

EP - 867

JO - Arthritis and Rheumatism

JF - Arthritis and Rheumatism

IS - 5

ER -

Detection of anticentromere antibodies using recombinant human CENP-A protein

Abstract

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