Cytogenetics of porcine sperm chromosomes following IVF of zona-free hamster oocytes

J. M. Bird, J. A. Houghton

Research output: Contribution to a Journal (Peer & Non Peer)Articlepeer-review

4 Citations (Scopus)

Abstract

Porcine sperm chromosome karyotypes were obtained from two boars following heterologous in vitro fertilization (IVF) of zona-free hamster ova with ionophore-capacitated porcine spermatozoa. The karyotype yield per inseminated ovum was 9.3% in = 1658), and there was no significant deviation from the expected 1 : 1 sex ratio (p > 0.5). Cytogenetic analysis revealed hyperhaploid (2.5% and hypohaploid spermatozoa (1.9% Spermatozoa with structural chromosome aberrations constituted 7.1% of the analyzed karyotypes, and these were predominantly chromosomal breaks, although acentric fragments were also found (2.6%

Original languageEnglish
Pages (from-to)45-57
Number of pages13
JournalSystems Biology in Reproductive Medicine
Volume25
Issue number1
DOIs
Publication statusPublished - 1990

Keywords

  • Chromosomes
  • Cytogenetics
  • Hamster
  • Oocyte
  • Pig
  • Sperm
  • Zona-free

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