Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development

Elaine D. Brabazon; Ronan T. Bree; Micheal W. Carton; Maura Grealy; Lucy Byrnes

doi:10.1016/S0167-4781(02)00302-0

Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development

Elaine D. Brabazon, Ronan T. Bree, Micheal W. Carton, Maura Grealy, Lucy Byrnes

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

4 Citations (Scopus)

Abstract

Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development.

Original language	English
Pages (from-to)	203-208
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1576
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-4781(02)00302-0
Publication status	Published - 7 Jun 2002

Keywords

CDNA
Cyclin-dependent kinase 8
Embryogenesis
Expression
Mid-blastula transition
Zebrafish

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10.1016/S0167-4781(02)00302-0

Cite this

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title = "Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development",

abstract = "Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development.",

keywords = "CDNA, Cyclin-dependent kinase 8, Embryogenesis, Expression, Mid-blastula transition, Zebrafish",

author = "Brabazon, {Elaine D.} and Bree, {Ronan T.} and Carton, {Micheal W.} and Maura Grealy and Lucy Byrnes",

year = "2002",

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doi = "10.1016/S0167-4781(02)00302-0",

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pages = "203--208",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

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Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development. / Brabazon, Elaine D.; Bree, Ronan T.; Carton, Micheal W. et al.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1576, No. 1-2, 07.06.2002, p. 203-208.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

TY - JOUR

T1 - Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development

AU - Brabazon, Elaine D.

AU - Bree, Ronan T.

AU - Carton, Micheal W.

AU - Grealy, Maura

AU - Byrnes, Lucy

PY - 2002/6/7

Y1 - 2002/6/7

N2 - Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development.

AB - Cyclin-dependent kinase 8 (cdk8) regulates transcription by phosphorylating RNA polymerase II and TFIIH. The mechanism of zygotic transcription activation during vertebrate embryonic development is poorly understood. Here we describe the cloning and developmental expression pattern of zebrafish cdk8 mRNA. It is highly conserved, sharing 79% DNA and 95% amino acid sequence identity with human cdk8, thereby indicating an important role for the protein. Northern blotting and whole mount in situ hybridisation revealed expression of zebrafish cdk8 maternally, following the onset of zygotic transcription at the mid-blastula transition (MBT) and throughout embryonic development.

KW - CDNA

KW - Cyclin-dependent kinase 8

KW - Embryogenesis

KW - Expression

KW - Mid-blastula transition

KW - Zebrafish

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U2 - 10.1016/S0167-4781(02)00302-0

DO - 10.1016/S0167-4781(02)00302-0

M3 - Article

SN - 0167-4781

VL - 1576

SP - 203

EP - 208

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1-2

ER -

Cyclin-dependent kinase 8 is expressed both maternally and zygotically during zebrafish embryo development

Abstract

Keywords

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