Abstract
Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.
| Original language | English (Ireland) |
|---|---|
| Pages (from-to) | 215-219 |
| Number of pages | 5 |
| Journal | Biochemical Journal |
| Volume | 386 |
| Issue number | Pt 2 |
| DOIs | |
| Publication status | Published - 1 Mar 2005 |
Keywords
- Epidermal growth factor (EGF)
- Glutathione
- Redox signalling
- Thioredoxin-1
- Thioredoxin-2
Authors (Note for portal: view the doc link for the full list of authors)
- Authors
- Halvey PJ, Watson WH, Hansen JM, Go YM, Samali A, Jones DP
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