Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture

Abhay Shashikant Pandit; Timothy O'Brien

doi:10.1038/srep20922

Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture

University of Galway

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33 Citations (Scopus)

Abstract

Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-beta 1). In tendon tissue repair, increased activity of TGF-beta 1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-beta 1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-beta 1 and a range of pro-fibrotic extracellular matrix genes.

Original language	English (Ireland)
Article number	20922
Number of pages	0
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep20922
Publication status	Published - 1 Feb 2016

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Authors
Abbah, SA,Thomas, D,Browne, S,O'Brien, T,Pandit, A,Zeugolis, DI
Abbah, SA;Thomas, D;Browne, S;O'Brien, T;Pandit, A;Zeugolis, DI

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10.1038/srep20922

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title = "Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture",

abstract = "Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-beta 1). In tendon tissue repair, increased activity of TGF-beta 1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-beta 1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-beta 1 and a range of pro-fibrotic extracellular matrix genes.",

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N2 - Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-beta 1). In tendon tissue repair, increased activity of TGF-beta 1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-beta 1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-beta 1 and a range of pro-fibrotic extracellular matrix genes.

AB - Extracellular matrix synthesis and remodelling are driven by increased activity of transforming growth factor beta 1 (TGF-beta 1). In tendon tissue repair, increased activity of TGF-beta 1 leads to progressive fibrosis. Decorin (DCN) and interleukin 10 (IL-10) antagonise pathological collagen synthesis by exerting a neutralising effect via downregulation of TGF-beta 1. Herein, we report that the delivery of DCN and IL-10 transgenes from a collagen hydrogel system supresses the constitutive expression of TGF-beta 1 and a range of pro-fibrotic extracellular matrix genes.

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Co-transfection of decorin and interleukin-10 modulates pro-fibrotic extracellular matrix gene expression in human tenocyte culture

Abstract

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