Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms

Ali Heydari; Nick D. Kim; Patrick J. Biggs; Jacqui Horswell; Gerty J. H. P. Gielen; Alma Siggins; Matthew D. Taylor; Collette Bromhead; Barry R. Palmer

doi:10.13025/21742

Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms

Ali Heydari
, Nick D. Kim
, Patrick J. Biggs
, Jacqui Horswell
, Gerty J. H. P. Gielen
, Alma Siggins
, Matthew D. Taylor
, Collette Bromhead
, Barry R. Palmer

Sustainable World

Massey University Wellington
Massey University
SCION
Waikato Regional Council

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

5 Citations (Scopus)

Abstract

Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.

Original language	English
Article number	772
Journal	Antibiotics
Volume	12
Issue number	4
DOIs	https://doi.org/10.13025/21742 https://doi.org/10.3390/antibiotics12040772
Publication status	Published - Apr 2023

Keywords

PICT
TRFLP
antibiotic resistance
bacteria
co-selection
heavy metal resistance
soil
trace elements

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10.13025/21742Licence: CC BY-NC-ND
10.3390/antibiotics12040772

2023 Antibiotics (Ali)Accepted author manuscript, 2.4 MBLicence: CC BY-NC-ND

Cite this

@article{a656511b488c4b4194168d44ae474cb0,

title = "Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms",

abstract = "Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.",

keywords = "PICT, TRFLP, antibiotic resistance, bacteria, co-selection, heavy metal resistance, soil, trace elements",

author = "Ali Heydari and Kim, \{Nick D.\} and Biggs, \{Patrick J.\} and Jacqui Horswell and Gielen, \{Gerty J. H. P.\} and Alma Siggins and Taylor, \{Matthew D.\} and Collette Bromhead and Palmer, \{Barry R.\}",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = apr,

doi = "10.13025/21742",

language = "English",

volume = "12",

journal = "Antibiotics",

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T1 - Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms

AU - Heydari, Ali

AU - Kim, Nick D.

AU - Biggs, Patrick J.

AU - Horswell, Jacqui

AU - Gielen, Gerty J. H. P.

AU - Siggins, Alma

AU - Taylor, Matthew D.

AU - Bromhead, Collette

AU - Palmer, Barry R.

PY - 2023/4

Y1 - 2023/4

N2 - Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.

AB - Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.

KW - PICT

KW - TRFLP

KW - antibiotic resistance

KW - bacteria

KW - co-selection

KW - heavy metal resistance

KW - soil

KW - trace elements

UR - http://hdl.handle.net/10379/17849

UR - https://www.scopus.com/pages/publications/85153728406

U2 - 10.13025/21742

DO - 10.13025/21742

M3 - Article

SN - 2079-6382

VL - 12

JO - Antibiotics

JF - Antibiotics

IS - 4

M1 - 772

ER -

Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms

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Ali Heydari

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Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms

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Ali Heydari

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