Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters; Patrick G. Murray; Yuta Miki; Angel T. Martínez; Maria G. Tuohy; Craig B. Faulds

doi:10.1128/AEM.05659-11

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters
, Patrick G. Murray
, Yuta Miki
, Angel T. Martínez
, Maria G. Tuohy
, Craig B. Faulds

Sustainable World

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

10 Citations (Scopus)

Abstract

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

Original language	English
Pages (from-to)	3759-3762
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	10
DOIs	https://doi.org/10.1128/AEM.05659-11
Publication status	Published - May 2012

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10.1128/AEM.05659-11

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title = "Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii",

abstract = "The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.",

author = "Waters, \{Deborah M.\} and Murray, \{Patrick G.\} and Yuta Miki and Mart{\'i}nez, \{Angel T.\} and Tuohy, \{Maria G.\} and Faulds, \{Craig B.\}",

year = "2012",

month = may,

doi = "10.1128/AEM.05659-11",

language = "English",

volume = "78",

pages = "3759--3762",

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publisher = "American Society for Microbiology",

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Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii. / Waters, Deborah M.; Murray, Patrick G.; Miki, Yuta et al.
In: Applied and Environmental Microbiology, Vol. 78, No. 10, 05.2012, p. 3759-3762.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

TY - JOUR

T1 - Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

AU - Waters, Deborah M.

AU - Murray, Patrick G.

AU - Miki, Yuta

AU - Martínez, Angel T.

AU - Tuohy, Maria G.

AU - Faulds, Craig B.

PY - 2012/5

Y1 - 2012/5

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AB - The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

UR - https://www.scopus.com/pages/publications/84861125185

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DO - 10.1128/AEM.05659-11

M3 - Article

SN - 0099-2240

VL - 78

SP - 3759

EP - 3762

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Abstract

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