Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana

Lokesh Joshi; Raymond J. St. Leger; Michael J. Bidochka

doi:10.1016/0378-1097(94)00500-Q

Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana

Lokesh Joshi, Raymond J. St. Leger, Michael J. Bidochka

Boyce Thompson Institute at Cornell University

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

74 Citations (Scopus)

Abstract

A Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA clone of the protease was isolated from mycelia of B. bassiana grown on cuticle/chitin cultures. The amino acid sequence of this gene was compared to that of Metarhizium anisopliae Pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase K, isolated from Tritirachium album, a saprophytic fungus. The cDNA sequence revealed that B. bassiana Prl is synthesized as a large precursor (M_r 37 460) containing a signal peptide, a propeptide and the mature protein predicted to have an M_r of 26 832.

Original language	English
Pages (from-to)	211-217
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	125
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(94)00500-Q
Publication status	Published - 15 Jan 1995
Externally published	Yes

Keywords

Beauveria bassiana
Beauveria bassiana Pr1
Cuticle degradation
DNA sequence
Protease gene

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10.1016/0378-1097(94)00500-Q

Cite this

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title = "Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana",

abstract = "A Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA clone of the protease was isolated from mycelia of B. bassiana grown on cuticle/chitin cultures. The amino acid sequence of this gene was compared to that of Metarhizium anisopliae Pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase K, isolated from Tritirachium album, a saprophytic fungus. The cDNA sequence revealed that B. bassiana Prl is synthesized as a large precursor (Mr 37 460) containing a signal peptide, a propeptide and the mature protein predicted to have an Mr of 26 832.",

keywords = "Beauveria bassiana, Beauveria bassiana Pr1, Cuticle degradation, DNA sequence, Protease gene",

author = "Lokesh Joshi and \{St. Leger\}, \{Raymond J.\} and Bidochka, \{Michael J.\}",

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doi = "10.1016/0378-1097(94)00500-Q",

language = "English",

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T1 - Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana

AU - Joshi, Lokesh

AU - St. Leger, Raymond J.

AU - Bidochka, Michael J.

PY - 1995/1/15

Y1 - 1995/1/15

N2 - A Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA clone of the protease was isolated from mycelia of B. bassiana grown on cuticle/chitin cultures. The amino acid sequence of this gene was compared to that of Metarhizium anisopliae Pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase K, isolated from Tritirachium album, a saprophytic fungus. The cDNA sequence revealed that B. bassiana Prl is synthesized as a large precursor (Mr 37 460) containing a signal peptide, a propeptide and the mature protein predicted to have an Mr of 26 832.

AB - A Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA clone of the protease was isolated from mycelia of B. bassiana grown on cuticle/chitin cultures. The amino acid sequence of this gene was compared to that of Metarhizium anisopliae Pr1, the only pathogenicity determinant so far described from an entomopathogenic fungus, and proteinase K, isolated from Tritirachium album, a saprophytic fungus. The cDNA sequence revealed that B. bassiana Prl is synthesized as a large precursor (Mr 37 460) containing a signal peptide, a propeptide and the mature protein predicted to have an Mr of 26 832.

KW - Beauveria bassiana

KW - Beauveria bassiana Pr1

KW - Cuticle degradation

KW - DNA sequence

KW - Protease gene

UR - https://www.scopus.com/pages/publications/0028985647

U2 - 10.1016/0378-1097(94)00500-Q

DO - 10.1016/0378-1097(94)00500-Q

M3 - Article

SN - 0378-1097

VL - 125

SP - 211

EP - 217

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

Cloning of a cuticle-degrading protease from the entomopathogenic fungus, Beauveria bassiana

Abstract

Keywords

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