Cloning, characterization and chromosomal location of a satellite DNA from the pacific oyster, Crassostrea gigas

Catherine Clabby; Usha Goswami; Fiona Flavin; Noel P. Wilkins; James A. Houghtona; Richard Powell

doi:10.1016/0378-1119(95)00749-0

Cloning, characterization and chromosomal location of a satellite DNA from the pacific oyster, Crassostrea gigas

Catherine Clabby
, Usha Goswami
, Fiona Flavin
, Noel P. Wilkins
, James A. Houghtona
, Richard Powell

Zoology

University of Galway
National Institute of Oceanography India
John Radcliffe Hospital

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

52 Citations (Scopus)

Abstract

We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster, Crassostrea gigas (Cg). The monomeric unit was found to be 166 (± 2) bp in length with 79-94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1-4% of the Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.

Original language	English
Pages (from-to)	205-209
Number of pages	5
Journal	Gene
Volume	168
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00749-0
Publication status	Published - 12 Feb 1996

Keywords

Genomic content
HaeIII and FokI repeated DNA
Heterochromatic location
Sequence analysis
Tandem repetition

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10.1016/0378-1119(95)00749-0

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title = "Cloning, characterization and chromosomal location of a satellite DNA from the pacific oyster, Crassostrea gigas",

abstract = "We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster, Crassostrea gigas (Cg). The monomeric unit was found to be 166 (± 2) bp in length with 79-94\% homology between monomers of the array. The sequence is A+T-rich (60\%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1-4\% of the Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.",

keywords = "Genomic content, HaeIII and FokI repeated DNA, Heterochromatic location, Sequence analysis, Tandem repetition",

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doi = "10.1016/0378-1119(95)00749-0",

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T1 - Cloning, characterization and chromosomal location of a satellite DNA from the pacific oyster, Crassostrea gigas

AU - Clabby, Catherine

AU - Goswami, Usha

AU - Flavin, Fiona

AU - Wilkins, Noel P.

AU - Houghtona, James A.

AU - Powell, Richard

PY - 1996/2/12

Y1 - 1996/2/12

N2 - We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster, Crassostrea gigas (Cg). The monomeric unit was found to be 166 (± 2) bp in length with 79-94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1-4% of the Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.

AB - We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster, Crassostrea gigas (Cg). The monomeric unit was found to be 166 (± 2) bp in length with 79-94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1-4% of the Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.

KW - Genomic content

KW - HaeIII and FokI repeated DNA

KW - Heterochromatic location

KW - Sequence analysis

KW - Tandem repetition

UR - https://www.scopus.com/pages/publications/0030038408

U2 - 10.1016/0378-1119(95)00749-0

DO - 10.1016/0378-1119(95)00749-0

M3 - Article

C2 - 8654945

AN - SCOPUS:0030038408

SN - 0378-1119

VL - 168

SP - 205

EP - 209

JO - Gene

JF - Gene

IS - 2

ER -

Cloning, characterization and chromosomal location of a satellite DNA from the pacific oyster, Crassostrea gigas

Abstract

Keywords

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