Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA

Janet Davidson; Terry Smith; Samuel A.M. Martin

doi:10.1006/fsim.1999.0222

Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA

Janet Davidson
, Terry Smith
, Samuel A.M. Martin

Infectious Disease

University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

14 Citations (Scopus)

Abstract

A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254bp and had an open reading frame of 648bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84·4% identity with medaka (Oryzias latipes) to 61·4% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24h. A 3·7-fold increase in mRNA was observed between control and stimulated cells.

Original language	English
Pages (from-to)	621-632
Number of pages	12
Journal	Fish and Shellfish Immunology
Volume	9
Issue number	8
DOIs	https://doi.org/10.1006/fsim.1999.0222
Publication status	Published - Nov 1999

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Proteasome, LMP2, mRNA expression, rainbow trout, cDNA sequence, Oncorhynchus mykiss L.

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10.1006/fsim.1999.0222

Cite this

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title = "Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA",

abstract = "A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254bp and had an open reading frame of 648bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84·4\% identity with medaka (Oryzias latipes) to 61·4\% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24h. A 3·7-fold increase in mRNA was observed between control and stimulated cells.",

keywords = "Proteasome, LMP2, mRNA expression, rainbow trout, cDNA sequence, Oncorhynchus mykiss L.",

author = "Janet Davidson and Terry Smith and Martin, \{Samuel A.M.\}",

year = "1999",

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doi = "10.1006/fsim.1999.0222",

language = "English",

volume = "9",

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journal = "Fish and Shellfish Immunology",

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TY - JOUR

T1 - Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA

AU - Davidson, Janet

AU - Smith, Terry

AU - Martin, Samuel A.M.

PY - 1999/11

Y1 - 1999/11

N2 - A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254bp and had an open reading frame of 648bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84·4% identity with medaka (Oryzias latipes) to 61·4% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24h. A 3·7-fold increase in mRNA was observed between control and stimulated cells.

AB - A Rainbow trout Oncorhynchus mykiss (L.) clone encoding the low molecular weight proteasome LMP 2 has been cloned and sequenced from rainbow trout leukocyte cells that had been stimulated with the mitogen phytohemagglutinin (PHA). The cDNA was 1254bp and had an open reading frame of 648bp encoding 217 amino acids. The sequence has a high degree of identity with other published LMP 2 sequences varying from 84·4% identity with medaka (Oryzias latipes) to 61·4% identity with Xenopus laevis. Northern blot analysis showed the LMP 2 mRNA to be expressed in a wide range of tissues, with two transcripts in gill, spleen and anterior kidney RNA. Up regulation of the LMP 2 mRNA transcript was shown in cells that were maintained in primary culture following stimulation with the PHA mitogen for 24h. A 3·7-fold increase in mRNA was observed between control and stimulated cells.

KW - Proteasome, LMP2, mRNA expression, rainbow trout, cDNA sequence, Oncorhynchus mykiss L.

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U2 - 10.1006/fsim.1999.0222

DO - 10.1006/fsim.1999.0222

M3 - Article

SN - 1050-4648

VL - 9

SP - 621

EP - 632

JO - Fish and Shellfish Immunology

JF - Fish and Shellfish Immunology

IS - 8

ER -

Cloning and sequence analysis of rainbow trout LMP 2 cDNA and differential expression of the mRNA

Abstract

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