Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution

Lucy Byrnes; Chi Cheng Luo; Wen Hsiung Li; Chao yuh Yang; Lawrence Chan

doi:10.1016/0006-291X(87)91137-5

Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution

Lucy Byrnes
, Chi Cheng Luo
, Wen Hsiung Li
, Chao yuh Yang
, Lawrence Chan

Baylor College of Medicine
The University of Texas Health Science Center at Houston

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

28 Citations (Scopus)

Abstract

Using an antibody against chicken apolipoprotein (apo) A-I, we identified multiple cDNA clones for the protein in two intestinal cDNA libraries in λgtll. The complete nucleotide sequence of chicken apoA-I cDNA was determined. The sequence predicts a mature protein of 240 amino acids, a 6-amino acid propeptide and an 18-amino acid signal peptide. Using a ³²P-cDNA probe, we detected the presence of apoA-I mRNA in 21 day old chicken intestine, liver, kidney, spleen, breast muscle and brain. The primary sequence of apoA-I contains numerous tandem repeats of 11 and 22 residues in a manner similar to the mammalian proteins. Our analysis of apoA-I sequences from human, rabbit, dog, rat, and chicken indicates that the rate of amino acid substitution is considerably faster in the rat lineage than in other mammalian lineages.

Original language	English
Pages (from-to)	485-492
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	148
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(87)91137-5
Publication status	Published - 14 Oct 1987
Externally published	Yes

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title = "Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution",

abstract = "Using an antibody against chicken apolipoprotein (apo) A-I, we identified multiple cDNA clones for the protein in two intestinal cDNA libraries in λgtll. The complete nucleotide sequence of chicken apoA-I cDNA was determined. The sequence predicts a mature protein of 240 amino acids, a 6-amino acid propeptide and an 18-amino acid signal peptide. Using a 32P-cDNA probe, we detected the presence of apoA-I mRNA in 21 day old chicken intestine, liver, kidney, spleen, breast muscle and brain. The primary sequence of apoA-I contains numerous tandem repeats of 11 and 22 residues in a manner similar to the mammalian proteins. Our analysis of apoA-I sequences from human, rabbit, dog, rat, and chicken indicates that the rate of amino acid substitution is considerably faster in the rat lineage than in other mammalian lineages.",

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T1 - Chicken apolipoprotein A-I

T2 - cDNA sequence, tissue expression and evolution

AU - Byrnes, Lucy

AU - Luo, Chi Cheng

AU - Li, Wen Hsiung

AU - Yang, Chao yuh

AU - Chan, Lawrence

PY - 1987/10/14

Y1 - 1987/10/14

N2 - Using an antibody against chicken apolipoprotein (apo) A-I, we identified multiple cDNA clones for the protein in two intestinal cDNA libraries in λgtll. The complete nucleotide sequence of chicken apoA-I cDNA was determined. The sequence predicts a mature protein of 240 amino acids, a 6-amino acid propeptide and an 18-amino acid signal peptide. Using a 32P-cDNA probe, we detected the presence of apoA-I mRNA in 21 day old chicken intestine, liver, kidney, spleen, breast muscle and brain. The primary sequence of apoA-I contains numerous tandem repeats of 11 and 22 residues in a manner similar to the mammalian proteins. Our analysis of apoA-I sequences from human, rabbit, dog, rat, and chicken indicates that the rate of amino acid substitution is considerably faster in the rat lineage than in other mammalian lineages.

AB - Using an antibody against chicken apolipoprotein (apo) A-I, we identified multiple cDNA clones for the protein in two intestinal cDNA libraries in λgtll. The complete nucleotide sequence of chicken apoA-I cDNA was determined. The sequence predicts a mature protein of 240 amino acids, a 6-amino acid propeptide and an 18-amino acid signal peptide. Using a 32P-cDNA probe, we detected the presence of apoA-I mRNA in 21 day old chicken intestine, liver, kidney, spleen, breast muscle and brain. The primary sequence of apoA-I contains numerous tandem repeats of 11 and 22 residues in a manner similar to the mammalian proteins. Our analysis of apoA-I sequences from human, rabbit, dog, rat, and chicken indicates that the rate of amino acid substitution is considerably faster in the rat lineage than in other mammalian lineages.

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DO - 10.1016/0006-291X(87)91137-5

M3 - Article

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AN - SCOPUS:0023653467

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Chicken apolipoprotein A-I: cDNA sequence, tissue expression and evolution

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