TY - JOUR
T1 - Chemical structure of a polysaccharide from Campylobacter jejuni 176.83 (serotype O:41) containing only furanose sugars
AU - Hanniffy, Orla M.
AU - Shashkov, Alexander S.
AU - Moran, Anthony P.
AU - Prendergast, Martina M.
AU - Senchenkova, Sof'ya N.
AU - Knirel, Yuriy A.
AU - Savage, Angela V.
PY - 1999/6/30
Y1 - 1999/6/30
N2 - A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained β-L-arabinose, 6-deoxy-β-D-altro-heptose (β-D-6d-altHep) and 6-deoxy-β-L-altrose (β-L-6d-Alt), whereas in the minor trisaccharide the β-L-6d-Alt is replaced by its C-5 epimer α-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established:→2)-β-L-Araf-(1→2)-β-D-6d-altHepf-(1→2)-β-L-6d-Altf-(1→ (75%)→2)-β-L-Araf-(1→2)-β-D-6d-altHepf-(1→2)-α-D-Fucf-(1→ (25%) Copyright (C) 1999 Elsevier Science Ltd.
AB - A neutral polysaccharide was obtained by hot phenol-water extraction of biomass from Campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential GPC on Bio-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. The major trisaccharide contained β-L-arabinose, 6-deoxy-β-D-altro-heptose (β-D-6d-altHep) and 6-deoxy-β-L-altrose (β-L-6d-Alt), whereas in the minor trisaccharide the β-L-6d-Alt is replaced by its C-5 epimer α-D-Fuc. On the basis of 1H and 13C NMR spectroscopic studies, including 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of the repeating units were established:→2)-β-L-Araf-(1→2)-β-D-6d-altHepf-(1→2)-β-L-6d-Altf-(1→ (75%)→2)-β-L-Araf-(1→2)-β-D-6d-altHepf-(1→2)-α-D-Fucf-(1→ (25%) Copyright (C) 1999 Elsevier Science Ltd.
KW - 6-Deoxy-D-altro-heptose
KW - 6-Deoxy-L-altrose
KW - Campylobacter jejuni
KW - D-Fucose
KW - L-Arabinose
KW - NMR spectroscopy
KW - O:41 Polysaccharide, structure
UR - https://www.scopus.com/pages/publications/0032871061
U2 - 10.1016/S0008-6215(99)00129-9
DO - 10.1016/S0008-6215(99)00129-9
M3 - Article
SN - 0008-6215
VL - 319
SP - 124
EP - 132
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 1-4
ER -