Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners

Caitríona E. McInerney; A. Louise Allcock; Mark P. Johnson; Paulo A. Prodöhl

doi:10.1007/s10592-008-9750-7

Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners

Caitríona E. McInerney
, A. Louise Allcock
, Mark P. Johnson
, Paulo A. Prodöhl

Zoology

Queen's University of Belfast
University of Galway

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

8 Citations (Scopus)

Abstract

Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per locus was 11 (range 4-29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively. Loci did not deviate from Hardy-Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies.

Original language	English
Pages (from-to)	1417-1420
Number of pages	4
Journal	Conservation Genetics
Volume	10
Issue number	5
DOIs	https://doi.org/10.1007/s10592-008-9750-7
Publication status	Published - Sept 2009

Keywords

Enrichment
Gastropod
Littorina
Littorina littorea
Microsatellite
Population genetics

Access to Document

10.1007/s10592-008-9750-7

Cite this

@article{637d7bb0601b4504a7b715f747127376,

title = "Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners",

abstract = "Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per locus was 11 (range 4-29), and observed and expected heterozygosities ranged from 6.9 to 84.3\% and from 9.4 to 95.2\%, respectively. Loci did not deviate from Hardy-Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies.",

keywords = "Enrichment, Gastropod, Littorina, Littorina littorea, Microsatellite, Population genetics",

author = "McInerney, \{Caitr{\'i}ona E.\} and Allcock, \{A. Louise\} and Johnson, \{Mark P.\} and Prod{\"o}hl, \{Paulo A.\}",

year = "2009",

month = sep,

doi = "10.1007/s10592-008-9750-7",

language = "English",

volume = "10",

pages = "1417--1420",

journal = "Conservation Genetics",

issn = "1566-0621",

publisher = "Springer Science and Business Media B.V.",

number = "5",

}

Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners. / McInerney, Caitríona E.; Allcock, A. Louise; Johnson, Mark P. et al.
In: Conservation Genetics, Vol. 10, No. 5, 09.2009, p. 1417-1420.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

TY - JOUR

T1 - Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners

AU - McInerney, Caitríona E.

AU - Allcock, A. Louise

AU - Johnson, Mark P.

AU - Prodöhl, Paulo A.

PY - 2009/9

Y1 - 2009/9

N2 - Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per locus was 11 (range 4-29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively. Loci did not deviate from Hardy-Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies.

AB - Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per locus was 11 (range 4-29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively. Loci did not deviate from Hardy-Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies.

KW - Enrichment

KW - Gastropod

KW - Littorina

KW - Littorina littorea

KW - Microsatellite

KW - Population genetics

UR - https://www.scopus.com/pages/publications/70350555516

U2 - 10.1007/s10592-008-9750-7

DO - 10.1007/s10592-008-9750-7

M3 - Article

SN - 1566-0621

VL - 10

SP - 1417

EP - 1420

JO - Conservation Genetics

JF - Conservation Genetics

IS - 5

ER -

Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this