CENP-I is essential for centromere function in vertebrate cells

Ai Nishihashi; Tokuko Haraguchi; Yasushi Hiraoka; Toshimichi Ikemura; Vinciane Regnier; Helen Dodson; William C. Earnshaw; Tatsuo Fukagawa

doi:10.1016/S1534-5807(02)00144-2

CENP-I is essential for centromere function in vertebrate cells

Ai Nishihashi, Tokuko Haraguchi, Yasushi Hiraoka, Toshimichi Ikemura, Vinciane Regnier, Helen Dodson, William C. Earnshaw, Tatsuo Fukagawa

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

117 Citations (Scopus)

Abstract

We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.

Original language	English
Pages (from-to)	463-476
Number of pages	14
Journal	Developmental Cell
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/S1534-5807(02)00144-2
Publication status	Published - 2002
Externally published	Yes

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title = "CENP-I is essential for centromere function in vertebrate cells",

abstract = "We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.",

author = "Ai Nishihashi and Tokuko Haraguchi and Yasushi Hiraoka and Toshimichi Ikemura and Vinciane Regnier and Helen Dodson and Earnshaw, {William C.} and Tatsuo Fukagawa",

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language = "English",

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TY - JOUR

T1 - CENP-I is essential for centromere function in vertebrate cells

AU - Nishihashi, Ai

AU - Haraguchi, Tokuko

AU - Hiraoka, Yasushi

AU - Ikemura, Toshimichi

AU - Regnier, Vinciane

AU - Dodson, Helen

AU - Earnshaw, William C.

AU - Fukagawa, Tatsuo

PY - 2002

Y1 - 2002

N2 - We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.

AB - We identified a novel essential centromere protein, CENP-I, which shows sequence similarity with fission yeast Mis6 protein, and we showed that CENP-I is a constitutive component of the centromere that colocalizes with CENP-A, -C, and -H throughout the cell cycle in vertebrate cells. To determine the precise function of CENP-I, we examined its role in centromere function by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-I, cells arrested at prometaphase with misaligned chromosomes for long periods of time. Eventually, cells exited mitosis without undergoing cytokinesis. Immunocytochemical analysis of CENP-I-deficient cells demonstrated that both CENP-I and CENP-H are necessary for localization of CENP-C but not CENP-A to the centromere.

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U2 - 10.1016/S1534-5807(02)00144-2

DO - 10.1016/S1534-5807(02)00144-2

M3 - Article

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VL - 2

SP - 463

EP - 476

JO - Developmental Cell

JF - Developmental Cell

IS - 4

ER -

CENP-I is essential for centromere function in vertebrate cells

Abstract

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