TY - JOUR
T1 - CD30 expression on T lymphocytes reflects disease activity in atopic asthma
AU - Leonard, Colm
AU - Tormey, V.
AU - Faul, J.
AU - Burke, C.
AU - Poulter, L. W.
PY - 1996/10
Y1 - 1996/10
N2 - PURPOSE: CD30, a protein expressed on and shed from activated T lymphocytes, is a marker of Th2 phenotype (producing interleukin-4 and 5). We examined whether serum CD30 and CD30 surface expression could define differences between atopic asthma, atopic non-asthma and normal individuals and whether CD30 was related to disease activity. METHODS: We measured serum CD30 in atopic asthmatics, atopic non-asthmatics, non-atopic asthma and normal individuals using an Elisa assay. We cultured peripheral blood mononuclear cells from atopic asthmatics, atopic non-asthmatics and normals, stimulated them with IL-2 and house dust mite antigen and assayed CD30 expression on T lymphocytes after 10 days using flow cytometry. RESULTS: Mean serum CD30 levels in atopic asthmatics (X = 149 i.u. / ml, N = 62), non-atopic asthmatics (X = 107 i.u. / ml, N = 13) and atopic non asthmatics (X = 90 i.u. / ml, N = 36) were significantly higher than normal individuals (X = 14 i.u. / ml, N = 9). The cell cultures revealed that on day 10 CD30 expression on CD4 lymphocytes was significantly higher in atopic asthmatics (X = 25.2% N = 6) compared to normals (X = 0.73 %, N = 4), p = 0.006. Atopic non-asthmatics had lower levels of CD30 expression (X = 10.5 %, N = 4) than atopic asthmatics but this was not significant at p < 0.05. In the atopic asthmatics there was a positive correlation between CD30 expression and symptoms. CONCLUSIONS: Serum CD30 is elevated in atopic patients and asthmatics (including non-atopic asthmatics) compared to normal CD30 expression on CD4 cells in response to house dust mite was highest in atopic asthmatics and correlated with disease activity and symptoms. CLINICAL IMPLICATIONS: CD30 may be a useful clinical and research tool for assessing disease severity in atopic asthma and monitoring response to immunotherapy. As viruses are the most potent inducers of CD30 expression high serum CD30 levels in non-atopic asthmatics may support a viral aetiology in these patients.
AB - PURPOSE: CD30, a protein expressed on and shed from activated T lymphocytes, is a marker of Th2 phenotype (producing interleukin-4 and 5). We examined whether serum CD30 and CD30 surface expression could define differences between atopic asthma, atopic non-asthma and normal individuals and whether CD30 was related to disease activity. METHODS: We measured serum CD30 in atopic asthmatics, atopic non-asthmatics, non-atopic asthma and normal individuals using an Elisa assay. We cultured peripheral blood mononuclear cells from atopic asthmatics, atopic non-asthmatics and normals, stimulated them with IL-2 and house dust mite antigen and assayed CD30 expression on T lymphocytes after 10 days using flow cytometry. RESULTS: Mean serum CD30 levels in atopic asthmatics (X = 149 i.u. / ml, N = 62), non-atopic asthmatics (X = 107 i.u. / ml, N = 13) and atopic non asthmatics (X = 90 i.u. / ml, N = 36) were significantly higher than normal individuals (X = 14 i.u. / ml, N = 9). The cell cultures revealed that on day 10 CD30 expression on CD4 lymphocytes was significantly higher in atopic asthmatics (X = 25.2% N = 6) compared to normals (X = 0.73 %, N = 4), p = 0.006. Atopic non-asthmatics had lower levels of CD30 expression (X = 10.5 %, N = 4) than atopic asthmatics but this was not significant at p < 0.05. In the atopic asthmatics there was a positive correlation between CD30 expression and symptoms. CONCLUSIONS: Serum CD30 is elevated in atopic patients and asthmatics (including non-atopic asthmatics) compared to normal CD30 expression on CD4 cells in response to house dust mite was highest in atopic asthmatics and correlated with disease activity and symptoms. CLINICAL IMPLICATIONS: CD30 may be a useful clinical and research tool for assessing disease severity in atopic asthma and monitoring response to immunotherapy. As viruses are the most potent inducers of CD30 expression high serum CD30 levels in non-atopic asthmatics may support a viral aetiology in these patients.
UR - https://www.scopus.com/pages/publications/33750268986
M3 - Article
AN - SCOPUS:33750268986
SN - 0012-3692
VL - 110
SP - 39S
JO - Chest
JF - Chest
IS - 4 SUPPL.
ER -
