CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)

Dearbhaile Morris; Martin Cormican

doi:10.1016/j.scitotenv.2016.04.075

CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)

Bacteriology

University of Galway
National Federation of Group Water Schemes

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

10 Citations (Scopus)

Abstract

Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30 L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens. (c) 2016 Elsevier B.V. All rights reserved.

Original language	English (Ireland)
Pages (from-to)	267-272
Number of pages	6
Journal	Science Of The Total Environment
Volume	563
DOIs	https://doi.org/10.1016/j.scitotenv.2016.04.075
Publication status	Published - 1 Sep 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being
SDG 6 Clean Water and Sanitation

Keywords

Filtration
Real time PCR
Verocytotoxigenic E. coli
Water

Authors (Note for portal: view the doc link for the full list of authors)

Authors
Morris, D,Kavanagh, S,Carney, K,MacDomhnaill, B,Cormican, M

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10.1016/j.scitotenv.2016.04.075

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Dive into the research topics of 'CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)'. Together they form a unique fingerprint.

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Morris, D., & Cormican, M. (2016). CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC). Science Of The Total Environment, 563, 267-272. https://doi.org/10.1016/j.scitotenv.2016.04.075

Morris, Dearbhaile ; Cormican, Martin. / CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC) : A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC). In: Science Of The Total Environment. 2016 ; Vol. 563. pp. 267-272.

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abstract = "Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30 L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens. (c) 2016 Elsevier B.V. All rights reserved.",

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language = "English (Ireland)",

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Morris, D & Cormican, M 2016, 'CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)', Science Of The Total Environment, vol. 563, pp. 267-272. https://doi.org/10.1016/j.scitotenv.2016.04.075

CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC). / Morris, Dearbhaile ; Cormican, Martin.
In: Science Of The Total Environment, Vol. 563, 01.09.2016, p. 267-272.

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

TY - JOUR

T1 - CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)

T2 - A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)

AU - Morris, Dearbhaile

AU - Cormican, Martin

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30 L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens. (c) 2016 Elsevier B.V. All rights reserved.

AB - Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30 L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens. (c) 2016 Elsevier B.V. All rights reserved.

KW - Filtration

KW - Real time PCR

KW - Verocytotoxigenic E. coli

KW - Water

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U2 - 10.1016/j.scitotenv.2016.04.075

DO - 10.1016/j.scitotenv.2016.04.075

M3 - Article

VL - 563

SP - 267

EP - 272

JO - Science Of The Total Environment

JF - Science Of The Total Environment

ER -

Morris D , Cormican M. CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC). Science Of The Total Environment. 2016 Sep 1;563:267-272. doi: 10.1016/j.scitotenv.2016.04.075

CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC)

Abstract

UN SDGs

Keywords

Authors (Note for portal: view the doc link for the full list of authors)

Access to Document

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Cite this