CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC).

Dearbhaile Morris

doi:10.1016/j.scitotenv.2016.04.075

CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC).

Dearbhaile Morris

Bacteriology

Research output: Contribution to a Journal (Peer & Non Peer) › Article › peer-review

Abstract

Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens.

Original language	Undefined/Unknown
Journal	The Science of the total environment
DOIs	https://doi.org/10.1016/j.scitotenv.2016.04.075
Publication status	Published - 30 Apr 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Access to Document

10.1016/j.scitotenv.2016.04.075

Cite this

@article{fb1aa3f88dce4da5b9b39f3d913d01c3,

title = "CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC).",

abstract = "Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens.",

author = "Dearbhaile Morris",

year = "2016",

month = apr,

day = "30",

doi = "10.1016/j.scitotenv.2016.04.075",

language = "Undefined/Unknown",

journal = "The Science of the total environment",

}

TY - JOUR

T1 - CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC).

AU - Morris, Dearbhaile

PY - 2016/4/30

Y1 - 2016/4/30

N2 - Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens.

AB - Verocytotoxigenic Escherichia coli (VTEC) is associated with a wide spectrum of disease from mild self-limiting diarrhoea to haemolytic uremic syndrome. Contaminated drinking water is accepted as an important route of transmission in Ireland as elsewhere however established methods for detection of VTEC in drinking water have limitations. We describe a sensitive and rapid method for detection of VTEC from large volumes (20 to 30L) of drinking water based on filtration, enrichment culture of filters and real-time PCR detection of VTEC virulence and O antigen determinants from enrichments. The method has potential applications for other waterborne pathogens.

UR - https://doi.org/10.1016/j.scitotenv.2016.04.075

U2 - 10.1016/j.scitotenv.2016.04.075

DO - 10.1016/j.scitotenv.2016.04.075

M3 - Article

C2 - 27135590

JO - The Science of the total environment

JF - The Science of the total environment

ER -

CapE (capture, amplify, extract): A rapid method for detection of low level contamination of water with Verocytotoxigenic Escherichia coli (VTEC).

Abstract

UN SDGs

Access to Document

Other files and links

Cite this