TY - JOUR
T1 - Biogas-based denitrification in a biotrickling filter
T2 - Influence of nitrate concentration and hydrogen sulfide
AU - López, Juan C.
AU - Porca, Estefanía
AU - Collins, Gavin
AU - Pérez, Rebeca
AU - Rodríguez-Alija, Alberto
AU - Muñoz, Raúl
AU - Quijano, Guillermo
N1 - Publisher Copyright:
© 2016 Wiley Periodicals, Inc.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - The feasibility of NO3 − removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3 − concentration on N2O production during this process was also evaluated. The results showed that NO3 − was removed at rates up to 2.8 g mreactor −3 h−1 using CH4 as electron donor. N2O production rates correlated with NO3 − concentration in the liquid phase, with a 10-fold increase in N2O production as NO3 − concentration increased from 50 to 200 g m−3. The use of H2S as co-electron donor resulted in a 13-fold increase in NO3 − removal rates (∼18 gNO3 − m−3 h−1) and complete denitrification under steady-state conditions, which was supported by higher abundances of narG, nirK, and nosZ denitrifying genes. Although the relative abundance of the DAMO population in the consortium was reduced from 60% to 13% after H2S addition, CH4 removals were not compromised and H2S removal efficiencies of 100% were achieved. This study confirmed (i) the feasibility of co-oxidising CH4 and H2S with denitrification, as well as (ii) the critical need to control NO3 − concentration to minimize N2O production by anoxic denitrifiers. Biotechnol. Bioeng. 2017;114: 665–673.
AB - The feasibility of NO3 − removal by the synergistic action of a prevailing denitrifying anoxic methane oxidising (DAMO), and nitrate-reducing and sulfide-oxidising bacterial (NR-SOB) consortium, using CH4 and H2S from biogas as electron donors in a biotrickling filter was investigated. The influence of NO3 − concentration on N2O production during this process was also evaluated. The results showed that NO3 − was removed at rates up to 2.8 g mreactor −3 h−1 using CH4 as electron donor. N2O production rates correlated with NO3 − concentration in the liquid phase, with a 10-fold increase in N2O production as NO3 − concentration increased from 50 to 200 g m−3. The use of H2S as co-electron donor resulted in a 13-fold increase in NO3 − removal rates (∼18 gNO3 − m−3 h−1) and complete denitrification under steady-state conditions, which was supported by higher abundances of narG, nirK, and nosZ denitrifying genes. Although the relative abundance of the DAMO population in the consortium was reduced from 60% to 13% after H2S addition, CH4 removals were not compromised and H2S removal efficiencies of 100% were achieved. This study confirmed (i) the feasibility of co-oxidising CH4 and H2S with denitrification, as well as (ii) the critical need to control NO3 − concentration to minimize N2O production by anoxic denitrifiers. Biotechnol. Bioeng. 2017;114: 665–673.
KW - anoxic hydrogen sulfide oxidation
KW - biotrickling filter
KW - denitrification
KW - denitrifying anoxic methane oxidation
KW - nitrate removal
KW - nitrous oxide production
UR - http://www.scopus.com/inward/record.url?scp=84992446826&partnerID=8YFLogxK
U2 - 10.1002/bit.26092
DO - 10.1002/bit.26092
M3 - Article
C2 - 27596480
AN - SCOPUS:84992446826
SN - 0006-3592
VL - 114
SP - 665
EP - 673
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 3
ER -