Assessment of basic biological functions exerted by miRNAs

John Nolan; Raymond L. Stallings; Olga Piskareva

doi:10.1007/978-1-4939-6524-3_2

Assessment of basic biological functions exerted by miRNAs

John Nolan
, Raymond L. Stallings
, Olga Piskareva

Royal College of Surgeons in Ireland
Our Ladies Hospital for Sick Children

Research output: Chapter in Book or Conference Publication/Proceeding › Chapter › peer-review

3 Citations (Scopus)

Abstract

Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	11-16
Number of pages	6
DOIs	https://doi.org/10.1007/978-1-4939-6524-3_2
Publication status	Published - 2017
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1509
ISSN (Print)	1064-3745

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell viability
Proliferation
Transfection
miRNA

Access to Document

10.1007/978-1-4939-6524-3_2

Cite this

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title = "Assessment of basic biological functions exerted by miRNAs",

abstract = "Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.",

keywords = "Cell viability, Proliferation, Transfection, miRNA",

author = "John Nolan and Stallings, \{Raymond L.\} and Olga Piskareva",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",

year = "2017",

doi = "10.1007/978-1-4939-6524-3\_2",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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booktitle = "Methods in Molecular Biology",

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TY - CHAP

T1 - Assessment of basic biological functions exerted by miRNAs

AU - Nolan, John

AU - Stallings, Raymond L.

AU - Piskareva, Olga

PY - 2017

Y1 - 2017

N2 - Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.

AB - Assessment of cell viability and proliferation under different miRNA expression levels is an important step in the evaluation of basic miRNA functional effects within the cell. Here, we describe the overexpression of miRNA in question in cells achieved by transfection with subsequent examination of cell viability and proliferation over a period of time using the acid phosphatase assay.

KW - Cell viability

KW - Proliferation

KW - Transfection

KW - miRNA

UR - https://www.scopus.com/pages/publications/84994877212

U2 - 10.1007/978-1-4939-6524-3_2

DO - 10.1007/978-1-4939-6524-3_2

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 11

EP - 16

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Assessment of basic biological functions exerted by miRNAs

Abstract

Publication series

UN SDGs

Keywords

Access to Document

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